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Sigma Aldrich Fine Chemicals Biosciences Protein A SepharoseR CL 4B
Supplier: Sigma Aldrich Fine Chemicals Biosciences GE17078001
Protein A Sepharose(R) CL-4B is a classic well documented medium for immunoglobin purification and fractionation and is suitable for immunoprecipitation procedures.Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose(R) CL-4B has been used as a powerful tool to isolate and purify classes subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding the Fab region is available for binding antigen.
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