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Apexbio Technology LLC Proteinase K 5ml
Supplier: Apexbio Technology LLC K10375
Proteinase K is a broad-spectrum serine protease Our product is derived from recombinant Pichia pastoris strains expressing the endoproteinase gene originally sourced from the fungus Tritirachium album limber It is a highly active enzyme widely used in the hydrolysis of a variety of proteins and enzymatic contaminants (including endonucleases exonucleases DNases and RNases) Proteinase K is therefore commonly used for DNA preparations as it does not impair DNA integrity Proteinase K preferentially cleaves peptide bonds adjacent to the carboxyl end of hydrophobic amino acids (aliphatic aromatic etc ) and exhibits excellent performance under various conditions such as different pH values buffer types detergents (e g SDS) chelating agents (e g EDTA) and temperatures Proteinase K is also used for enzyme mapping and the removal of unwanted enzymes from DNA preparations to enhance cloning efficiency The suitable working concentration usually ranges from 0 05 to 1 mg/mL
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