Sigma Aldrich Fine Chemicals Biosciences Proteinase K recombinant P

Supplier:  Sigma Aldrich Fine Chemicals Biosciences 3115801001

Proteinase K is a subtilisin-related serine protease. The recombinant enzyme is identical to the native protease originally isolated from the mold Tritirachium album. The specifications of the recombinant enzyme are the same as those of the native protease. The amino acid sequence (molecular weight) and the molecule structure are identical. However the recombinant preparation is much purer than the native enzyme as it is DNA-free that has no pronounced cleavage specificity. The enzyme is extremely effective on native proteins and can therefore be used to rapidly inactivate endogenous nucleases such as RNases and DNases. The enzyme is also available as a SOLUTIONon.Inhibitors The enzyme is inactivated by Pefabloc(R) SC. However it is not inactivated by metal ions chelating agents (e.g. EDTA) sulfhydryl reagents or trypsin and chymotrypsin inhibitors.Activators Proteinase K activity is stimulated by the presence of denaturing agents (SDS and urea).pH Optimum Proteinas

Catalog No. 50-290-5738