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ILEX LIFE SCIENCES LLC RAT FGF2 146AA E.COLI 10 UG
Supplier: ILEX LIFE SCIENCES LLC A36080010
Ilex Life Sciences FGF-2 (FGF-basic) Rat (146 aa), E. coli Recombinant Protein (10 ug). DESCRIPTION: Our bioactive FGF-2 (FGF-basic) Rat, E. coli Recombinant Protein (146 aa), also known as truncated Fibroblast Growth Factor 2, is a single, non-glycosylated, polypeptide chain with 146 amino acids and a 16.4 kDa molecular mass. The FGF2 is purified by proprietary chromatographic techniques. APPLICATION: FGF basic is a critical component of hESCs culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state. FORMULATION: Sterile filtered white powder lyophilized from 1 mg/ml solution after extensive dialysis against 10mM Na2PO4, pH 7.5 and 50mM NaCl. PURITY: Greater than 95.0% (SDS-PAGE). BIOACTIVITY: The ED50 range as determined by the dose-dependent proliferation of NR6R-3T3 cells was found to be 0.903 ng/ml, corresponding to a specific activity of 1.1x107 units/mg. SYNONYMS: FGF-b, Basic fibroblast growth factor, bFGF, HBGF-2.
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