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Supply Solutions Red Blood Cell Lysing Buffer Hybri-Max™, 100mL

Supplier:  Supply Solutions SIGMA R7757100ML

Encompass_Preferred

Recommended for use by adding 1 mL of buffer to a cell pellet (cell pellet = 1 spleen or 100-200 million cells). Gently mix for 1 minute. Dilute the buffer with 15-20 mL of medium or salt solution. Centrifuge at 250-500 g for 7 minutes and decant the supernatant. Cells may be diluted and prepared for counting or fusion. If lysis is incomplete, steps 1-4 may be repeated.

Catalog No. NC0829166


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