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ABclonal Technology Taq DNA Ligase
Supplier: ABclonal Technology RK2050210000U
Taq DNA Ligase is a thermostable ligase that catalyzes the formation of a phosphodiester bond between the 5?-phosphate and the 3?-hydroxyl of two adjacent DNA strands. The target strands need to be hybridized and accurately paired, with no gap, to a complementary DNA strand; allowing resolution of single nucleotide variants. Taq DNA Ligase uses NAD as a cofactor and it is active at elevated temperatures (37°C-75°C). It is applicable to Allele-specific gene detection using Ligase Detection Reaction and Ligase Chain Reaction, as well as Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification.
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