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ABclonal Technology LbCas12a Nuclease
Supplier: ABclonal Technology RK205701000PMOL
Lb Cas12a Nuclease from Lachnospiraceae bacterium is a site-specific DNA endonuclease guided by a single 40-44 nucleotide guide RNA (crRNA). Targeting requires a crRNA complementary to the target site as well as a 5’ TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by LbCas12a Nuclease occurs ~17 bases 3′ of the PAM and leaves overhanging ends.This product also has a trans-cleavage activity, which produces high non-specific ssDNA cleavage activity when the target dsDNA, crRNA, and Cas12a protein form a ternary complex.
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