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ABclonal Technology Fast Magnetic DNA Methylation Bisulfite Kit

Supplier: ABclonal Technology RK3020618RXN
This kit quickly converts unmethylated cytosines in DNA samples to uracil while leaving methylated cytosines unchanged. After bisulfite treatment at high temperature, double-stranded DNA is denatured into single strands. Under the action of HSO3-, cytosine residues undergo deamination and are converted to uracil, while methylated residues remain intact. Subsequently, during PCR amplification, uracil is replaced by thymine (T).
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