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Sigma Aldrich Fine Chemicals Biosciences LiberaseTM DH Research Gra
Supplier: Sigma Aldrich Fine Chemicals Biosciences LIBDHRO
The most commonly used method for decellularization procedures such as tissue dissociation and cell harvesting is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.Liberase enzyme technology comprises methods for purifying clostridial collagenase isoforms to high specific activity and for blending them together with high-specific-activity neutral protease in optimal ratios for effective dissociation of primary tissues and cultured cells. Liberase DH Research Grade contains highly purified Collagenase I and Collagenase II. These two collagenase isoforms are blended in a precise ratio to each other and with a high concentration of Dispase(R) a non-clostridial neutral protease.Liberase enzymes are designed to increase the quality and reprod
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