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IBA LifeSciences StrGate Acptr Vctr pASG-IBA143
Supplier: IBA LifeSciences 54143001
The pASG-IBA143 vector is designed for expression of recombinant proteins with an N-terminal His-tag and C-terminal Twin-Strep-tag in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, and the inducible tetracycline promoter/operator for the regulated expression of proteins. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.
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