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IBA LifeSciences StrGate Acptr Vctr pESG-IBA102
Supplier: IBA LifeSciences 54502001
The pESG-IBA102 vector is designed for high-level, stable, and non-replicative transient expression of target proteins with a C-terminal Twin-Strep-tag in mammalian cells. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells as well as the F1 and ColE1 origin for a high plasmid copy number. In addition, it carries the human cytomegalovirus (CMV) immediate-early promoter for high-level expression, the neomycin resistance gene for selection of stable cell lines, and the SV40 ori for episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. HEK293T, COS-1, COS-7). BM40 secretory signal peptide is encoded for the transfer of the expressed protein into the medium. During translocation from the cytosol the signal peptide is removed from the protein. In addition to the direct cloning of the gene of interest into pESG-IBA vectors with Esp3I, another option via Entry Vector is possible.
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