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Apexbio Technology LLC T7 Endonuclease I(Synonyms: T7EI, T7 Endo I, T7 Endonuclease 1, T7 Endo 1, T7 mismatch-specific endonuclease), 1250U.
Supplier: Apexbio Technology LLC K11061250
T7 Endonuclease I (T7EI) is a recombinant DNA endonuclease produced through expression in E coli T7EI specifically targets and cleaves diverse forms of DNA substrates containing structural anomalies including mismatches of at least two base pairs heteroduplex DNA cruciform structures Holliday junctions and branched DNA intermediates as well as nicked duplexes at reduced cleavage efficiency It hydrolyzes phosphodiester bonds located near mismatched nucleotides 5 ends This enzyme is widely employed in detecting genetic mutations induced by gene-editing systems (e g CRISPR/Cas9 TALENs) identifying single nucleotide polymorphisms (SNPs) and generating randomized DNA fragments for shotgun cloning techniques
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