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Sigma Aldrich Fine Chemicals Biosciences Terminal Transferase from
Supplier: Sigma Aldrich Fine Chemicals Biosciences 3333566001
Terminal Transferase catalyzes the template independent addition of deoxy- and dideoxynucleoside triphosphates to the 3-OH ends of double and single-stranded DNA fragments and oligonucleotides. Terminal Transferase incorporates digoxigenin- biotin- and fluorochrome-labeled deoxy- and dideoxynucleoside triphosphates as well as radioactively labeled deoxy- and dideoxynucleoside triphosphates. The supplied 5x-concentrated reaction buffer allows the optimal tailing of all types of double-stranded DNA ends blunt ended with 3 overhang or with 5 overhang. The highest incorporation rates are obtained with 3 overhangs.
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