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IBL America Testosterone (Free In Saliva) ELISA
Supplier: IBL America IB79316

Enzyme immunoassay (ELISA) for the determination of free active testosterone in saliva. For research use only, not for use in diagnostic procedures.An unknown amount of free testosterone presentin the sample and a defined amount of testosterone conjugated to horseradish peroxidase compete for thebinding sites of rabbit polyclonal testosterone antiserum coated to the wells of a microplate. After one-hourincubation on a shaker the microplate is washed four times. After addition of the substrate solution theconcentration of testosterone is inversely proportional to the optical density measured.
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