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ILEX LIFE SCIENCES LLC TRYPSINEDTA P10-036100 6X100ML
Supplier: ILEX LIFE SCIENCES LLC P10036100X6
PAN-Biotech Trypsin 0.25% / 0.53 mM EDTA in HBSS, w/o: Ca and Mg, w: Phenol Red (6 x 100 ml). DESCRIPTION: PAN-Biotech Trypsin 0.25% / 0.53 mM EDTA in HBSS, w/o: Ca and Mg, w: Phenol Red is a porcine pancreas-derived enzyme plus EDTA in HBSS (Hanks' Balanced Salt Solution). Trypsin-EDTA solution is commonly used for the dissociation of anchorage-dependent (adherent) mammalian cells and tissues from culture surfaces. The concentration of trypsin necessary to dislodge cells from their substrate varies with cell type and culture age. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: With Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. TRYPSIN: 0.25% Trypsin. EDTA: 0.53 mM EDTA. STERILE: Yes. STORAGE TEMP: -20°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
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