ECM Bioscience VE-Cadherin (Tyr-685), phospho-specific Rabbit Polyclonal Antibody, ECM Biosciences

Supplier:  ECM Bioscience CP1981

This antibody was cross-adsorbed to an unrelated phospho-tyrosine peptide and unphosphorylated VE-cadherin (Tyr-685) peptide before affinity purification using phospho-VE-cadherin (Tyr-685) peptide. The purified antibody detects a 140 kDa* band corresponding to VE-cadherin in western blots of human endothelial cells treated with pervanadate, and this band is not detected in untreated cells or after alkaline phosphatase treatment. The immunogen was Phospho-VE-Cadherin (Tyr-685) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding tyrosine 685 in human VE-cadherin. This sequence has significant homology to the conserved site in rat and mouse VE-cadherin, but is not conserved in other cadherins. Accession #P33151. Applications = WB, E; Species Reactivity = Hu, Rt, Ms; Size = 100 μl; Buffer = PBS, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year. Other Names = Cadherin-5, vascular endothelial Cadherin, CD144

Catalog No. 50-172-0721