Staining Reagents
INTACT GENOMICS INC ig 10B Electrocompetent Cells 12x100µl
Intact Genomics ig 10B (DH10B derivative) electrocompetent E. coli cells offer the highest transformation efficiencies of ≥5 x 1010 cfu/µg plasmid DNA which are ideal for applications requiring high transformation efficiencies, such as with cDNA or gDNA library construction.Product includes and Storage:ig 10B electroCompetent cells: -80 ºCpUC19 control DNA: -20 ºCRecovery medium: 4 ºC
Sigma Aldrich Fine Chemicals Biosciences BioTracker TiY Vimentin Li
Vimentin is an intermediate filament protein which is upregulated in cells undergoing epithelial-to-mesenchymal transition (EMT) and hence is commonly used an EMT marker. Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers including lung cancer.BioTracker TiY Vimentin is a live cell fluorescent dye that selectively stains TICs over differentiated tumor cells or normal cells. TiY can be used for visualization and enrichment of functionally active TICs from patient tumors. TiY displays better selectivity for tumorigenic cells than a CD166 antibody and can be used for TiY-staining-based cell sorting for TICs in various types of cancer (lung CNS melanoma breast renal ovarian colon and prostate cancer). At high concentrations TiY shows selective anti-TIC activity over non-TIC and normal tissue cells.Spectral PropertiesAbsorbance 553 nmEmission 573 nmReferencesLee Y et al. Identification of Tumor Initiating Cells w
Sigma Aldrich Fine Chemicals Biosciences BioTracker 555 Orange Cyto
Lipophilic carbocyanine dyes are widely used for labeling neurons in tissues by retrograde labeling and to label membranes in a wide variety of cell types. The dyes are weakly fluorescent in aqueous phase but become highly fluorescent in lipid bilayers. Staining is highly stable with low toxicity and very little dye transfer in between cells making the dyes suitable for long-term cell labeling and tracking studies. When live cells are stained the dyes label plasma membranes and also are taken up into endocytic compartments. Cells can be fixed either before or after staining although permeabilization affects the staining pattern.Unlike PKH dyes BioTracker Cytoplasmic Membrane Dyes do not require a complicated hypoosmotic labeling protocol. They are ready-to-use dye delivery SOLUTIONons that can be added directly to normal culture media to label suspended or adherent cells in culture. Spectral PropertiesAbsorbance 549nmEmission 565nm
Abcam Phenol red sodium salt, pH indicator, 25G
MW 376.37 g/mol. Water soluble pH indicator used in the 6.8 (yellow) to 8.2 (red) range. Commonly employed as a cell tissue culture pH marker. Under normal conditions, phenol red added to the growth medium will have a pink-red color. Dying cells or overgrowth of contaminants will cause a change in pH resulting in a change in indicator color.
The product is subject to the following: Abcam Restricted Use Statement
Electron Microscopy Sciences Tissue Marking Dye Kit, 7 dyes (7x2 oz)
Fast drying dye is ideal for marking tissue biopsies and pathology specimens and can be used with frozen or fixed tissues. It is a specially formulated binding pigment that evenly coats the tissue without penetrating the tissue surface. The marking dyes do not interfere with diagnostic interpretation due to the fact colors are not similar to standard histopathology dyes. It can be used to permanently mark the margins of excised surgical specimens for exact orientation.
American Research Products Inc BETA N ACETYLGLUCOSAMINIDASE N
5000265028 BETA N ACETYLGLUCOSAMINIDASE N
Electron Microscopy Sciences Mayer's Hematoxylin, 1L
Mayer's Hematoxylin is used as a counterstain for procedures such as immunohistochemistry or laser microdissection.