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Standard-value, reference solutions designed for the exact calibration of spectrometers; standards guarantee that machines function properly for accurate spectral lines.
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Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Under normal conditions PS resides on the inner side of the cell membrane lipid bilayer However in early apoptosis PS translocates from the inner leaflet of the plasma membrane to the outer surface becoming exposed externally Annexin V exhibits high binding affinity for PS and when conjugated with fluorescent molecules (Cy5) it serves as a fluorescent probe to detect apoptosis using flow cytometry or fluorescence microscopy The staining procedure requires only 10 20 minutes PI specifically binds to double-stranded DNA to produce red fluorescence it is normally impermeant to intact cell membranes However during late-stage apoptosis or in dead cells PI penetrates the membrane to stain nuclear DNA Combined Annexin V-Cy5 and PI staining allows distinction between cells at various stages of apoptosis and dead cells
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
If you are unable to find the chemical you are looking for, make sure you are logged into your fishersci.com account and click on the following link: eMolecules Building Block Tool<|a>
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More
Small and Specialty Supplier Partner Small and/or specialty supplier based on Federal laws and SBA requirements. Learn More
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) In normal conditions PS is located on the inner side of the cell membrane lipid bilayer However in the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the membrane becoming exposed to the extracellular environment Annexin V has a high affinity for PS and Annexin V labeled with Cy3 can serve as a fluorescent probe for detection of apoptotic cells using flow cytometry or fluorescence microscopy The staining procedure takes only 10 minutes
Encompass Procurement Services Non-distribution item offered as a customer accommodation; additional freight charges may apply. Learn More