Glycosyl compounds
Research Products International Corp p Nitro BD galactopyrano 50G
Colorimetric and spectrophotoametric substrate for detection of B-D Galactosidase.
Research Products International Corp p Nitro a D glucopyrano 50G
Spectrophotometer substrate for Glucansucrases.
Free PNP: <250ppm
Research Products International Corp TRIS HCl 10 KG
TRIS Hydrochloride, Tris-HCl, is a buffering agent commonly utilized to maintain a stable pH in experimental procedures.
It is primarily used as a buffering agent for applications that require a slightly alkaline to neutral pH range. Also, It is effective in the pH range of 7.2 to 9.0, making it suitable for a variety of biochemical and molecular biology techniques.
TRIS HCl is commonly used in DNA and RNA extraction, protein purification, enzyme assays, and electrophoresis. It helps maintain the desired pH conditions for enzymatic reactions, stability of nucleic acids, and separation of proteins during electrophoresis.
One of the advantages of TRIS HCl is its ability to resist changes in pH caused by the addition of acids or bases, making it a reliable buffering agent. It also exhibits low ionic strength, which minimizes interference with biological systems and enzymatic activities.
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-0.1.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 100 Milligrams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
![Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 5 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B71800-5.0.JPG-250.jpg)
Research Products International Corp X-GAL [5-Bromo-4-chloro-3-indolyl-B-D-galactoside], 5 Grams
Chromogenic substrate of β-Galactosidase used in combination with IPTG for detection of β-Galactosidase activity in bacterial colonies. X-GAL is cleaved at the B1-4 bond between galactose and the 5-Bromo-4-chloro-3-indolyl portion of X-GAL by β-Galactosidase via hydrolysis. The cleavage of X-GAL results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors such as Lambda-11, M13mp18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac+ cells. After transformation the cells show β-Galactosidase activity in the presence of media containing X-GAL and IPTG. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of β-Galactosidase activity leading to the appearance of white colonies. Non-recombinant cells produce a blue indigo dye.
Water Content: 0.05% (K. Fisher)
Protect from light and moisture
Research Products International Corp 4-Nitrophenyl-a-L-fucopyranoside, 100 Milligrams
Chromogenic substrate for detecting a-L-Fucosidase.
![Research Products International Corp [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-N81000-25.02.JPG-250.jpg)
Research Products International Corp [ONPG] 2-Nitrophenyl-B-D-Galactopyranoside, 25 Grams
Colorimetric and spectrophotometric substrate for detection of β-D-Galactosidase.
