Recombinant human interleukin-1 beta (IL-1β) with an N-terminal His tag, expressed in E. coli and supplied as a lyophilized powder. Intended for cell-based assays and biochemical studies, the protein is characterized for low endotoxin and defined biological activity, and is formulated for stability and long-term storage.
- Recombinant human IL-1β encompassing residues A117-S269.
- N-terminal His tag for purification and detection.
- Expressed in Escherichia coli and provided as lyophilized powder.
- Approx. 18 kDa molecular weight by SDS-PAGE.
- Endotoxin <1 EU/μg (LAL method).
- High specific activity (ED50 1.045 pg/mL in CTLL-2 assay).
- Formulated in 50 mM Tris-HCl, 300 mM NaCl, 200 mM arginine, pH 8.0.
- Store lyophilized at -20°C; reconstitute ≥100 μg/mL and add carrier for long-term storage.