Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld PEB- Protein Extraction Buffer (5x), 500 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Teknova 10mM Tris-HCl, pH 8.5. 2mL, 100-Pack, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 10mM Tris-HCl, pH 8.5, 15mL Tube, 25 Tubes per Pack, Sterile. 12 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Bioworld Tris-Triton Buffer, 500 mL
Tris-Triton bufferis the idealbuffer with detergent required to release membrane- or cytoskeleton- bound proteins.Contains: 10mM Tris (pH 7.4), 100mM NaCl, 1mM EDTA, 1mM EGTA, 1% Triton X-100, 10% Glycerol and 0.1% SDSAppearance: Liquid
Teknova Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Elution Solution-10mM Tris-HCl pH 8.5, 0.01% Tween-20. 1000mL. DNAse Tested. Sterile. 8 pack.
Biotang Inc HEPES Protein Extraction Buffer (5X), 250 ml
HEPES Protein Extraction Buffer (5X), 250 ml.
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eMolecules 895245-31-5 | trans-3,4-Bis-hydroxymethyl-pyrrolidine-1-carboxylic acid tert-butyl ester | J&W PharmLab LLC462.584 | C22H42N2O8 | 97.000 | CC(C)(C)OC(=O)N1C[C@H](CO)[C@@H](CO)C1.CC(C)(C)OC(=O)N1C[C@@H](CO)[C@H](CO)C1 | 1g | 592901059
trans-3,4-Bis-hydroxymethyl-pyrrolidine-1-carboxylic acid tert-butyl ester | J&W PharmLab LLC | 895245-31-5462.584 | C22H42N2O8 | 97.000 | CC(C)(C)OC(=O)N1C[C@H](CO)[C@@H](CO)C1.CC(C)(C)OC(=O)N1C[C@@H](CO)[C@H](CO)C1 | 1g | 592901059
Selleck Chemical LLC P62-mediated mitophagy inducer
P62-mediated mitophagy inducer (PMI) is a P62-mediated activator of mitophagy that functions independently of parkin recruitment or mitochondrial membrane potential ( m) collapse It remains effective even in cells lacking a fully functional PINK1/Parkin pathway PMI stabilizes Nrf2 and enhances p62 expression thereby activating mitophagy
eMolecules 415952-27-1 | methyl 4-((3-(hydroxymethyl)piperidin-1-yl)methyl)benzoate | Broadpharm | MFCD01452616 | 263.337 | C15H21NO3 | 97.000 | COC(=O)c1ccc(CN2CCCC(CO)C2)cc1 | 1g | 112541923
methyl 4-((3-(hydroxymethyl)piperidin-1-yl)methyl)benzoate | Broadpharm | 415952-27-1 | MFCD01452616 | 263.337 | C15H21NO3 | 97.000 | COC(=O)c1ccc(CN2CCCC(CO)C2)cc1 | 1g | 112541923