Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml
Cell crude extracts contain various endogenous enzymes such as phosphatases and proteases capable of degrading proteins present in the extracts The optimal strategy to enhance intact protein yield is adding specific inhibitors targeting these enzymes This Protease Inhibitor Cocktail is used in fungal and yeast extracts to improve protein stability by inhibiting proteases responsible for degrading both phosphorylated and non-phosphorylated protein substrates This Protease Inhibitor Cocktail consists of individual compounds including AEBSF 1 10-Phenanthroline E-64 and Pepstatin A which provide broad-spectrum specific inhibition of cysteine proteases serine proteases aspartic proteases and metalloproteases It is optimized and tested for fungal and yeast extracts This cocktail is available as a ready-to-use solution dissolved in DMSO
ILEX LIFE SCIENCES LLC EDTA 1P P10-026100 100 ML
PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg (100 ml). DESCRIPTION: PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 1.00% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC EDTA 1P P10-026100 6 X 100 ML
PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 1.00% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
![eMolecules TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | 1446012-48-1 | MFCD27986992 | 1g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502181707.JPG-250.jpg)
eMolecules TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | 1446012-48-1 | MFCD27986992 | 1g
AstaTech | TERT-BUTYL 6-(HYDROXYMETHYL)-5-OXA-2-AZASPIRO[3.4]OCTANE-2-CARBOXYLATE | 1g | 624127457 | P14281 | 95.000 | 1446012-48-1 | MFCD27986992 | 243.303 | C12H21NO4
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Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
Research Products International Corp TRIS, ACS Grade, 5 Kilograms
TRIS, also known as tris(hydroxymethyl)aminomethane, is a chemical compound widely used in life science as a buffer.
Teknova DNA Suspension Buffer, pH 8.0. 4L, Sterile, DNase/RNase tested, PCR Grade. 2 pack.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Apexbio Technology LLC One-step TUNEL Cy3 Apoptosis Detection Kit 50rxns
One-step TUNEL Cy3 Apoptosis Detection Kit can be used to detect nuclear DNA fragmentation occurring in tissues or cells during apoptosis Cellular DNA endonucleases are activated during apoptosis which cleave genomic DNA between nucleosomes yielding DNA fragments of approximately 180 - 200 bp or their multiples The resulting DNA fragments have exposed 3 -OH ends which can be labeled with a fluorescein-modified dUTP under the action of Terminal Deoxynucleotidyl Transferase (TdT) These labeled DNA fragments can then be detected using fluorescence microscopy or flow cytometry (Ex/Em) of 550 nm/570 nm This detection kit has a wide range of applications and can be used to assay apoptosis in frozen sections or paraffin-embedded tissue sections as well as cultured adherent or suspended cells
Bioworld TBS Buffer, Low Salt (10X), 4 L
Product DescriptionTris-buffered saline (TBS) is low salt used for Western blot washing with a concentration of 10X. It contains 500 mM Tris, 1.5M NaCl and has a pH level of 7.4.TBS is isotonic and non-toxic, and is used to dilute substances. This buffer is used as a washing buffer for electrodes and to wash microtiter plates for Western blot experiments and screening methods.
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | 5 MG
AR-13324 analog mesylate is an analog of AR-13324. This product is for research use only.
- Acts as a Rho-associated protein kinase (ROCK) inhibitor.
- Functions as a norepinephrine transporter (NET) inhibitor.