Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
CHEMSCIENCE Brass "A" for Sn, Pb, Cu, Zn
• This product is supplied in five lot of 500 g each of known concentration ( % concentration availiable upon request.)
• Detailed purity information, along with the Safety Data Sheet (SDS) and Technical Data Sheet (TDS), will be provided upon purchase.
Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 500 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-20 Bis-Tris Preca
mPAGE 4-20% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kd Medical Inc TBS, 20X, PH7.4, 2000ML
20X Tris Buffer Saline is ready for dilution to a 1X working solution. Used in various research applications. One 2 Liter cubitainer.
LAB BIOREAGENTS LowBinding V Buffer 500ml
Lab Bioreagents LowBindings (I, II, III, IV, V, VI, VII) are newly developed assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The LowBindings helps to reduce the non-specific bindings, negative effects of interfering molecules, and cross-reactivities of antibodies and proteins from biomatrix. Therefore, LowBindings exhibit the low matrix interference and high signal-to-noise, by which the assay sensitivity and specificity have been significantly improved. As many factors are involved in immunoassays, there is no single assay buffer that is ideal for each assay. LowBindings (I, II, III, IV, V, VI, VII) are a variety of buffers based on different formulations.
Selleck Chemical LLC P62-mediated mitophagy inducer
P62-mediated mitophagy inducer (PMI) is a P62-mediated activator of mitophagy that functions independently of parkin recruitment or mitochondrial membrane potential ( m) collapse It remains effective even in cells lacking a fully functional PINK1/Parkin pathway PMI stabilizes Nrf2 and enhances p62 expression thereby activating mitophagy
Research Products International Corp Potassium Phosphate, Monobasic, ACS Grade, 10 KG
Potassium Phosphate, Monobasic, ACS Grade is a chemical compound that holds significance in the life science research, particularly in molecular biology, cell culture, and biochemistry fields.
Alkali Scientific Murashige & Skoog Medium with MES Buffer and Vitamins, 245 Grams of Powder, Makes 50 Liters of Solution
Murashige & Skoog (MS) Medium with MES Buffer and Vitamins is a high-quality powdered formulation used for plant tissue culture. This 245g powder makes 50 liters of a nutrient-rich solution, providing all essential macronutrients, micronutrients, and growth hormones. The addition of MES buffer ensures a stable pH environment for long-term culture, while the B5 vitamins promote cell growth and differentiation. This medium is ideal for in vitro propagation, genetic transformation, and plant regeneration studies. It is widely used in plant biotechnology labs for various applications including cloning, gene editing, and somatic embryogenesis.
Bioworld BES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) Buffer, 0.5M, pH 6.5, 250 mL
Synonym: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid sodium salt, BES sodium saltBelongs to the class of Good buffers, as described in the research of Dr. Norman Good et al. (1966), optimized for molecular biology and biochemistry applications.The characteristics of a Good′s buffer include: pKa between 6.0 and 8.0, high solubility, non toxic, limited effect on biochemical reactions, very low absorbance between 240-700 nm, enzymatic and hydrolytic stability, minimal changes due to temperature and concentration, limited effects due to ionic or salt composition of the solution, limited interaction with mineral cations and limited permeability of biological membranes.Technical Information :pKa (25 °C) :7.1 (Free acid)Warning: For laboratory use only.Not fit for agricultural, household, clinical use or human consumption.Appearance: LiquidPH Value: 6.5
![Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660391.jpg-250.jpg)
Alkali Scientific DIPSO [3 (N,N Bis(2 Hydroxyethyl)amino) 2 hydroxypropane sulfonic acid], 25 G
DIPSO (3 [N,N-Bis(2 Hydroxyethyl)amino] 2-hydroxypropane sulfonic acid) is a biochemical reagent available in a 25g bottle. This buffering agent is widely used in molecular biology, biochemistry, and analytical chemistry for maintaining stable pH levels in low pH environments. DIPSO is ideal for enzyme assays, protein research, and chromatography, where maintaining a precise pH is critical. It is also useful in protein purification and electrophoresis, providing consistent and reliable results. The 25g size is perfect for smaller scale experiments or routine laboratory applications where a high-quality buffering solution is necessary for pH regulation.
FUJIFILM BIOSCIENCES INC RPMI Medium 1640 1X HEPES
RPMI Medium 1640 1X HEPES is designed for growth of humanLeukemia cells in either monolayers or suspension and is useful for a wide variety of suspension and monolayer cultures as well as for hybridomas