Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences CAPS | 1135-40-6 | MFCD00003837 | 250g
CAPS | Purity: 99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 250g
![Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661333.jpg-250.jpg)
Alkali Scientific HEPPSO [N (2 Hydroxyethyl) piperazine N' (2 hydroxypropanesulfonic acid)], 25 G
HEPPSO (N-(2 Hydroxyethyl) piperazine N'-(2 hydroxypropanesulfonic acid)) is a biochemical reagent available in a 25g bottle. This buffering agent is widely used in laboratory applications for maintaining pH stability in aqueous solutions. HEPPSO is ideal for use in biochemical research, including enzyme assays, protein purification, and other experiments requiring precise pH regulation. It helps maintain a stable pH environment in various biological assays, making it essential in molecular biology, cell culture, and electrophoresis. The 25g size makes it perfect for smaller-scale laboratory experiments where reliable buffering capacity is needed.
Bioworld ADA Buffer, 0.2M, pH 6.0, 100 mL
Product DescriptionADA (N-(2-acetamido)iminodiacetic acid) buffer has a concentration of 0.2M and a pH value of 6.0. It has a molecular weight of 190.2 and formula of C6H10N2O5. ADA is a zwitterionic buffer used in biochemistry and molecular biology, such as its use in protein-free medium for supporting chick embryo fibroblasts. It has also been shown to interfere with color development in bicinchoninic acid assay (BCA) for protein quantitation and used in isoelectric focusing of proteins in immobilized pH gradients.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld ACE MES Buffer, 50mM, pH 6.0, 250 mL
Product DescriptionA.C.E.™ MES (compound 2-(N-morpholino)ethanesulfonic acid) buffer has a concentration of 50 mM with a pH level of 6.0. The buffer is biotechnology grade and is formulated for capillary electrophoresis. Buffer is made with 3D water and filtered through 0.22 µ filter. Product is shipped at room temperature.
Selleck Chemical LLC HEPES
HEPES is a nonvolatile zwitterionic chemical buffering agent which is broadly applied in cell culture HEPES is effective at pH 6 8 to 8 2 HEPES is also a potent inducer of lysosome biogenesis
Hampton Research 100% TACSIMATE PH 8.0 200ML
HR2-829/100% Tacsimate pH 8.0 - 200 ml. Please note items from this supplier are non-returnable.
Research Products International Corp TRIS-Glycine-SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
Composition:
TRIS (Hydroxymethyl) Aminomethane: < 20.0%
SDS: < 6.0%
Bioworld Tris-Glycine Transfer Buffer (10X), 1 L
Tris-Glycine Transfer Buffer, 10X Concentrate.For Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.The 10× Tris-glycine buffer is diluted to 1× with methanol and water to make a solution containing 25 mM Tris, 192 mM glycine, and 20% methanol. A sufficient amount of transfer buffer should be made to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper.To make 1X Transfer Buffer from 10X concentrate: Mix 100 ml of 10X Transfer Buffer, 200 ml of methanol and 700 ml of dd water per liter.Foreign activity: Protease, none detected Storage temp.: 2-8°C
Electron Microscopy Sciences Phosphate Buffer 0.1M, pH 6.0 1 LT
Prepared fresh for each order from Sodium Phosphate Monobasic Hydrate and Sodium Phosphate Dibasic Heptahydrate. Non-Sterile. Meant for biological research only. Not to be used for medical or any other purposes.