Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | 1132-61-2 | MFCD00006183 | 1KG
MOPS anhydrous, free-flowing, Redi-Dri(TM), >=99.5% | Purity: >=99.5% | Mol Wt: 209.26 | 1132-61-2 | MFCD00006183 | 1KG
Sigma Aldrich Fine Chemicals Biosciences BICINE Redi DriTM 99 free 1KG
BICINE Redi DriTM 99 free 1KG
Research Products International Corp MOPS Sodium Salt 2.5 KG
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS Sodium Salt is its compatibility with biological systems and enzymes.
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 100 G
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and possesses a pKa of 7.84 at a 2.0 mM buffer concentration. It is intended for research use only and is not sold to patients.
- Working pH range of 7.1-8.5.
- Exhibits a relatively high ability to bind copper(II).
- Possesses a pKa of 7.84 at a 2.0 mM buffer concentration.
- For research use only.
- Molecular weight: 268.33.
- Formula: C9H20N2O5S.
- Appearance: Solid.
- Color: White to off-white.
Crescent Chemical Co Inc MORPHOLINOETHANE SULFONIC ACID
Morpholinoethane sulfonic acid analytical grade pKa 20 = 6.15. Buffering substance for biochemistry and molecular biology (1). Physical constants (2). Size - 500G Storage Conditions - +15 °C TO +30 °C Catalog Number - 29834.04 CAS 4432-31-9
Research Products International Corp HEPES Buffered Saline Solution 2X, 500 Milliliters
2X Formulation:
KCI: 10 mM
Na2HPO4: 1.5 mM
Dextrose: 12 mM
HEPES: 50 mM
pH: 7.0
![Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50255318.jpg-250.jpg)
Bioworld ACES (N-[carbamoylmethyl]-2-Aminoethane-sulfonic acid) Buffer, 0.5M, pH 7.0, 500 mL
Synonym:N-(2-Acetamido)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)-2-aminoethanesulfonic acid; N-(Carbamoylmethyl)taurine ACES is one ofGood's buffersdeveloped in the 1960s to provide buffers withpHranging from 6.15-8.35 for use in various applications. Azwitterionicbuffer with apKaof 6.9, it is often used as abuffering agent inbiologicalandbiochemicalresearch. ACES had been used to develop buffers for bothagaroseandpolyacrylamide gel electrophoresis.A saturated solution contains 0.22 M at 0°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+or Mn2+. Appearance: LiquidHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 7.0
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660042.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 250 G
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid) in a 250g volume is a biochemical reagent designed for pH buffering in biological and chemical experiments. MES is widely used for maintaining a stable pH in laboratory applications such as protein analysis, enzyme assays, and cell culture. This specific size is ideal for smaller volume experiments where larger quantities are unnecessary. MES provides a precise and consistent pH environment, making it invaluable in research involving electrophoresis, chromatography, and other biochemical processes. Its non-interfering nature with biological systems ensures that it does not affect the results of sensitive assays and experiments, especially in molecular biology.
![Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid] sodium salt, 3 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659870.jpg-250.jpg)
Alkali Scientific MES Sodium Salt [2 (N Morpholino)ethanesulfonic acid] sodium salt, 3 Kg
MES Sodium Salt [2-(N-Morpholino)ethanesulfonic acid] Sodium Salt, 3 Kg, is a powerful buffering agent used in various laboratory applications, particularly in the biological and chemical fields. This reagent helps maintain pH stability in experiments requiring a neutral to slightly acidic environment. MES is widely used in electrophoresis, enzyme assays, and cell culture media. With a capacity to buffer in the pH range of 5.5 to 6.7, it ensures reliable results in sensitive biological assays. The 3 kg package offers sufficient volume for ongoing experiments and provides an economical option for regular laboratory use in research and clinical applications.
AG Scientific Inc MOPS Free Acid 50 G
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions. The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.
Bioworld TAE Buffer, Quick-Mix Pack (10X), 4 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 10X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Crescent Chemical Co Inc PROTEIN TEST MIX 4 FOR SDS PG
Protein Test Mixture 4 for SDS PAGE 4 Dalton-marker proteins, high molecular weight, for SDS PAGE Molecular weight markers for SDS gel electrophoresis. The bovine albumin (BSA) contains monomers and oligomers.Reconstitute with SDS sample buffer (125 mM Tris-HCl pH 6.8, 2 % SDS, 15 %