Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld MOPS-SDS Running Buffer (20X), 1 L
MOPS-SDS Running Buffer for Protein Gel electrophoresisSDS running buffer with MOPS and Tris (pH 7.7)20X concentrationAll bioWORLD running buffers are prepared with high quality reagentsSpecifications:Ready to use, just dilute in distilled water. Save time and ensure reproducible results.1X contains: 50mM MOPS, 50mM Tris base, 1mM EDTA, and 0.1% SDS
Biotium Ready-to-Use Tyramide Amplification Buffer, 1X
Ready-to-use tyramide amplification buffer can be used for tyramide signal amplification with CF Dye Tyramides or other tyramides without the need to add hydrogen peroxide.
Quality Biological Inc TAE Buffer 50X, 10 Liters
TAE buffer provides faster electrophoretic migration of linear DNA and better resolution of supercoiled DNA. ¿ TAE Buffer may be more suitable than TBE, for electrophoresis if DNA is to be recovered from the agarose and purified using commercially available DNA purification kits. ¿ Applications: TAE buffer has a relatively low buffering capacity, therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase RNase and Protease Tested ¿ Concentration: 2 M Tris base and 0.05 M disodium EDTA dihydrate ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc TBS 10X pH 7.4, 10 Liters
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. ¿ DNase, RNase and Protease tested. ¿ Applications: TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be used in detergent preparation and Western blot or ELISA applications. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ pH: 7.4¿0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
eMolecules Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | 117550-36-4 | MFCD14680132 | 250mg
WuXi AppTec | Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | 250mg | 583186989 | LN01746856 | | 117550-36-4 | MFCD14680132 | 181.191 | C9H11NO3
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Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Sigma Aldrich Fine Chemicals Biosciences MOPSO sodium salt 99 titra500G
MOPSO sodium salt 99 titra500G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid purified grade, >=98.5%, powder | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid purified grade, >=98.5%, powder | Purity: >=98.5% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Boston Bioproducts Inc Tris Buffered Saline, TBS (20X, for Western Blot Washing) - 4L
The Tris Buffered Saline (TBS) is an isotonic non-toxic buffer solution used commonly in biochemical applications. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. Tris maintains a stable pH range typically between 7.4 to 8.0 while NaCl regulates osmotic balance via its elected concentration. This TBS Solution is formulated at a 20X concentration, and is useful for washing in Western Blotting.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.2 (0.2 micron filtered), 500 ml.
Aniara Diagnostica LLC PBS TBLT PH 7.4 1000ML 100 EA
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed tablets yields 1000 mL 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.