Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Pyridoxal which is an aldehyde has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.
Alkali Scientific CellPro™ Tris-HCL Buffer 1M, pH 10.2, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ MES Buffered Saline, 5X pH 6.5, 1L
Alkali Scientific™ CellPro™ MES Buffered Saline offers a meticulously prepared solution indispensable for a myriad of biochemical and molecular biology applications. This 5X concentrated buffer, with a pH of 6.5, is meticulously formulated to ensure optimal performance and reproducibility in diverse experimental setups. The MES (2-(N-morpholino)ethanesulfonic acid) buffer system provides stable pH conditions, making it ideal for various enzymatic reactions, protein assays, and nucleic acid studies. Its unique pH level of 6.5 suits specific experimental requirements, facilitating precise control over reaction conditions. With a generous volume of 1 liter, this CellPro™ MES Buffered Saline caters to the needs of laboratories conducting high-throughput assays or requiring ample buffer supply for extended experimental runs. Trust in the quality and consistency of CellPro™ MES Buffered Saline to support your research endeavors and enable robust and reliable results.
Alkali Scientific CellPro™ Tris Buffer-HCL, 1M, pH 8.0, 20L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ PBS Buffer, with Ca + Mg, 1X, pH 7.4, 500mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Quality Biological Inc PBS (10X), pH 7.4, 500 mL
Balanced salt solution ¿ Wash cells before dissociation ¿ Transport cells or tissue ¿ Dilute cells for counting ¿ Prepare reagents ¿ Endotoxin and Mycoplasma tested ¿ Useful in immunological applications, such as Western Blotting. ¿ Storage Condition: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ Endotoxin and Mycoplasma Tested ¿ Osmolality: 300 ¿ 55 mOsm/Kg H20 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ UNSPSC: 12161700 ¿ Max Shelf Life: 24 months
Quality Biological Inc TBS 10X pH 7.4, CS 10 X 1000 mL
TBS 10X pH 7.4 is used as a washing buffer for cellular and molecular biology techniques. ¿ DNase, RNase and Protease tested. ¿ Applications: TBS 10X pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS 10X pH 7.4 can also be used in detergent preparation and Western blot or ELISA applications. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase, and Protease Tested ¿ pH: 7.4¿0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc Tris Glycine Transfer Buffer 10X (Transblotting Buffer 10X), 1000ML
Tris Glycine Transfer Buffer at a 1X concentration with the addition of 20% methanol is used for electrophoretic transfer of proteins from denaturing polyacrylamide gel to a solid membrane ¿ i.e. Western blotting or Tris-glycine gel electrophoresis.
Quality Biological Inc Tris HCl (1 M), pH 7.5, 1000 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc Tris HCL (1 M), pH 8.0, 500 mL
A hybridization buffer specifically formulated for use with nylon membranes. ¿ It does not contain Denhardt¿s Reagent, which may interfere with the hybridization reaction of DNA immobilized on nylon. ¿ Formulated for Nylon membrane ¿ Filtered through a 0.22 ¿m membrane ¿ DNase, RNase, and Protease tested ¿ Applications: Denatured fragmented salmon sperm DNA may be added to this hybridization buffer to reduce nonspecific background. ¿ Storage Conditions: 25¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc Tris HCl (1 M), pH 7.5, 100 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Sigma Aldrich Fine Chemicals Biosciences MOPS-EDTA-Sodium Acetate M1L
MOPS - EDTA - Sodium Acetate (MESA) is the most commonly used running buffer for RNA electrophoresis prior to Northern blotting procedures. Applied voltage of 5 V/cm is recommended for maximum reSOLUTIONon.