Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 10X PBS-MK, 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 20X PBS. 20L, Sterile
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Alkali Scientific CellPro™ DPBS, 10X, 500mL
Experience superior performance and convenience with Alkali Scientific™ CellPro™ 10X DPBS, Dulbecco’s Phosphate Buffered Saline. This concentrated buffer solution offers ten times the strength of standard DPBS, providing researchers with a cost-effective and time-saving option for buffer preparation. With its precisely balanced formulation and high purity, CellPro™ 10X DPBS ensures consistent results in cell culture, molecular biology, and immunocytochemistry applications. Trust Alkali Scientific™ CellPro™ 10X DPBS for reliable performance and reproducibility in your laboratory workflows.
Electron Microscopy Sciences Pipes 1.5 Sodium Salt, Crystal, 6.8 @25°C pH 25 GR
(Piperazine-N,N-bis (2-ethanesulfonic acid,) 1.5 Sodium)
F.W. 335.3
pH: 6.8 @25°C
Medchemexpress LLC TAE Powder / TAE Buffer | 100 POUCHES
TAE (Tris-acetate-EDTA) buffer is widely used in biological experiments, especially in agarose gel electrophoresis of nucleic acid. Linear dsDNA migrates quickly in electrophoresis, and DNA fragments (> 13 kb) can be separated effectively. TAE buffer is also suitable for DNA gel extraction.
- Consists of Tris-acetate and EDTA-2Na.
- Each pouch can be diluted to 1 L of 1x TAE buffer.
- Concentration of Tris-acetate is 40 mM and EDTA-2Na is 1 mM when diluted to 1 L of 1x TAE buffer.
- Provides a convenient way to make TAE solution.
- Eliminates the need to weigh and mix individual components.
- pH of the 1x TAE buffer is 8.3 ± 0.1 @ 25°C.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M pH 7.0
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences PIPES 99 titration25KG
PIPES is a member of the ethanesulfonic acid buffer series first introduced by Good et al. developed to meet certain criteria midrange pKa maximum water solubility and minimum solubility in all other solvents minimal salt effects minimal change in pKa with temperature chemically and enzymatically stable minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 C is near physiological pH PIPES has applications in cell culture work.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Medchemexpress LLC PBS-T Powder | 100 POUCHES
MCE PBS-T Powder (1 L of 1x) is a formulation consisting of NaCl, KCl, Phosphate, and Tween 20. It is extensively utilized as a washing buffer in immunoassay experiments such as ELISA and Western Blot. Additionally, it can be employed for antibody dilution and the preparation of blocking solutions. The product is conveniently packaged, with 20 pouches defined as the base specification, and larger sizes offered as incremental volumes of this base. Each pouch is designed to be diluted to 1 L of 1x PBS-T buffer.
- Widely used in ELISA and Western Blot.
- Can be used for antibody dilution and preparation of blocking solution.
- Consists of NaCl, KCl, Phosphate, and Tween 20.
- Each pouch dilutes to 1 L of 1x PBS-T buffer.
- Eliminates the need to weigh and mix individual components.
- Convenient way to make PBS-T buffer.
- Final 1x PBS-T buffer has a pH of 7.4 ± 0.2 @ 25°C.
Electron Microscopy Sciences Hank's Balanced Salts Formula II 500 ML
All of our Hanks' Balanced Salt Solutions contain: Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Use the table below as a quick reference for identifying the one that best suits your application. All solutions are prepared in 1X strength.
Electron Microscopy Sciences D-PBS, Formula I Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Cayman Chemical TrIs 4 7dIphenyl1 10phen 25mg
A luminescent probe (absorption λmax: 455 nm, luminescence λmax: 613 nm) widely used for detection and quantitation of oxygen; strongly reduced by molecular oxygen as a result of dynamic quenching; thus oxygen detection can be based on either measurement of intensity or decay time
Kd Medical Inc PBS BUFFER, 1X, PH 7.2
Standard buffer for many biomolecular procedures. One 1 Liter bottle.