Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld TT - SDS Running Buffer (Cathode) (10X), 500 mL
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.3. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Kd Medical Inc TRIS BUFF SALINE,10X PH 7.6 1L
Commonly used buffer used as a wash buffer in multiple laboratory applications. 10X concentrated TBS is ready to be diluted to a 1X working solution. pH 7.6, one 1 Liter bottle.
eMolecules 174346-82-8 | tert-Butyl 2-(hydroxymethyl)azetidine-1-carboxylate | MFCD03790936 | 1g
Ambeed | 3-Chlorothiobenzamide | 5g | 600842207 | A516790 | 2548-79-0 | MFCD00276993 | 171.640 | C7H6ClNS
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Seracare Life Sciences Inc 10X TRIS-GLYCINE TRANS BUFFER
10X Tris-glycine Transfer Buffer is intended for Western blotting and gel electrophoresis. It is ideal for preparing a standard Western blot transfer buffer (Towbin) and for use as a gel electrophoresis buffer for native Tris-glycine gel without SDS. pH=8.3. Conductivity controlled.