Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-500.02.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 500 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Research Products International Corp PIPES SODIUM SALT, 100 GRAM
pKa (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Sigma Aldrich Fine Chemicals Biosciences PIPES 99 titration25KG
PIPES is a member of the ethanesulfonic acid buffer series first introduced by Good et al. developed to meet certain criteria midrange pKa maximum water solubility and minimum solubility in all other solvents minimal salt effects minimal change in pKa with temperature chemically and enzymatically stable minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 C is near physiological pH PIPES has applications in cell culture work.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M pH 7.0
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt 99.5 titr1KG
MOPS sodium salt 99.5 titr1KG
Promocell GmbH PromoCell Dulbeccos PBS pH 7,2-7,8 500 ml, Standard, with Ca++/ Mg++
Buffers and Salt Solutions are frequently used in tissue culture, cell isolation procedures, and molecular biology.For convenience, PromoCell offers ready-to-use Buffers and Salt Solutions. They are prepared from raw materials of the highest quality using tissue culture grade water.Dulbecco's PBS can be purchased with or without Ca^2 ^/ Mg^2 ^.