Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC HEPES hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 | C8H18N2O4S·1/2Na | 5 G
HEPES hemisodium salt is a zwitterionic buffering agent supplied as a dry powder for laboratory use. With high assay purity, it is used to maintain physiological pH in biochemical and cell culture applications and is intended for research use only. Store sealed and protected from moisture; solids are commonly refrigerated and prepared solutions can be frozen for longer-term storage.
- High purity (99.0% titration) suitable for analytical and cell culture work.
- Zwitterionic buffer effective in the pH 6.8-8.2 range.
- Supplied as a dry powder for flexible preparation of buffered solutions.
- Recommended storage: sealed, away from moisture, refrigerated for solid.
- In-solution stability: -20 °C (short term), -80 °C (long term) when stored sealed.
- Intended for research use only; not validated for clinical applications.
eMolecules Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4
Ambeed / 2-(Bis(2-hydroxyethyl)amino)acetic acid / 10g / 525008933 / A114701 / / 150-25-4 / MFCD00004295 / 163.173 / C6H13NO4
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 10 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659832.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 10 Kg
HEPES, Free Acid (N-(2-Hydroxyethyl) Piperazine-N'-(2-Ethanesulfonic Acid)) is a biochemical reagent that provides highly stable pH conditions for biological and chemical research. The 10kg bulk size is designed for large laboratories that require significant quantities of HEPES for buffer preparation and other research applications. HEPES is widely used in cell culture, protein assays, and enzyme reactions due to its minimal interference with biological processes. It is ideal for applications where precise pH control is essential, such as in molecular biology, immunology, and microbiology. The free acid form of HEPES is highly soluble in water, providing a versatile and effective buffer for a variety of laboratory procedures.
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661641.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 1 Kg
MES Monohydrate (2-(N-Morpholino) ethanesulfonic acid) is a biochemical reagent used for pH buffering in biochemical applications. This 1 kg volume is ideal for laboratories requiring large quantities of MES buffer for a variety of experiments. MES is commonly used in biochemical research, especially in enzyme assays, cell culture, and protein purification. It maintains a stable pH range, crucial for experiments that need precise and consistent conditions. The MES buffer ensures minimal interference in reactions, making it ideal for applications such as electrophoresis and chromatography. MES Monohydrate is a reliable and effective reagent used in a wide range of scientific studies, particularly in molecular biology and biochemistry.
Medchemexpress LLC MOPS (sodium salt) | 71119-22-7 | 99.8% | 1 KG
MOPS sodium salt is a buffering agent used in biology. It maintains the pH of mammalian cell culture media and significantly improves the thermal stability of BSA.
- Buffering agent for biological applications.
- Maintains pH in mammalian cell culture media.
- Improves thermal stability of BSA.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | 6976-37-0 | MFCD00002853 | 100G
BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, >=98.0% | Purity: >=98.0% | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 100G
eMolecules 7365-44-8 | TES | Oakwood Chemical | MFCD00007532 | 229.250 | C6H15NO6S | 99.000 | OCC(CO)(CO)NCCS(O)(=O)=O | 1g | 537724648
TES | Oakwood Chemical | 7365-44-8 | MFCD00007532 | 229.250 | C6H15NO6S | 99.000 | OCC(CO)(CO)NCCS(O)(=O)=O | 1g | 537724648
Sigma Aldrich Fine Chemicals Biosciences HEPES BioXtra pH 5.0 6.5 1250G
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.
Gold Biotechnology Inc MES Free Acid 100 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Chemscene ChemScene | Boc-Piperazine-OH | 25G | CS-W009135 | 0.98 | 77279-24-4| MFCD00728947 | 230.3
ChemScene | Boc-Piperazine-OH | 25G | CS-W009135 | 0.98 | 77279-24-4| MFCD00728947 | 230.3
Bioworld ACES Buffer, 0.5M, pH 7.5, 100 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
Boston Bioproducts Inc MOPS Buffer (1 M, pH 7.5) - 500ml
The MOPS Buffer [3- (N-morpholino)propanesulfonic acid buffer] is a versatile zwitterionic Good's buffer used in biological research. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH, and maintains a pH range from 6.5 to 7.9. It is often used as a buffering agent in biology and biochemistry, and is recommended for polyacrylamide gel electrophoresis, as a running buffer, in separating RNA in agarose gels, and for protein purification chromatography.
Bioworld BES-Buffered Saline (2X), 500 mL
Product DescriptionBES (N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid) saline buffer has a concentration of 2X and a pH of 7.1 at 25°C. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test. This saline buffer contains 50 mM BES, 1.5 mM Na2HPO4, and 280 mM NaCl.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
![Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M22040-250.02.JPG-250.jpg)
Research Products International Corp MES Monohydrate [2-(N-Morpholino)-ethanesulfonic acid], 250 Grams
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7