Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC NVP-TAE 684 | 761439-42-3 | 99.7% | 614.20 g/mol | C30H40ClN7O3S | 100 MG
NVP-TAE 684 is a potent, selective anaplastic lymphoma kinase (ALK) inhibitor used in research to block proliferation of ALCL-derived and ALK-dependent cell lines. It shows low-nanomolar activity and is supplied as a high-purity small molecule for in vitro studies and compound screening.
- Potent ALK inhibition with IC50 in the low-nanomolar range (≈2-10 nM).
- Selective activity against ALK-dependent and ALCL-derived cell lines.
- Suitable for in vitro research and compound screening applications.
- Available as solid and DMSO solution formats and multiple pack sizes.
- High purity suitable for research use (>99.7%).
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTA 5L
NC3626876 50 MM TRIS-HCL 10 MM EDTA 5L
Bioworld TTETRIS-TAPS-EDTA) BUFFER
This item has a minimum order quantity of 2 per supplier requirements. TTE (TRIS-TAPS-EDTA) BUFFER POWDER BLEND: - Proteomics Grade - An electrophoresis running buffer for DNA Sequencing that yields longer reads
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml.
eMolecules 5704-04-1 | Tricine | Oakwood Chemical | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Tricine | Oakwood Chemical | 5704-04-1 | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Alkali Scientific TRIS CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
TRIS CAPS Buffer 10X Solution is designed for use in capillary electrophoresis and other advanced electrophoretic techniques. This formulation, available in two 500mL bottles, ensures convenient preparation and consistent results during capillary separation of charged biomolecules. The 10X solution can be diluted to create the working concentration needed for various electrophoresis applications. TRIS CAPS buffer is particularly useful in separating proteins and nucleic acids in a high-resolution format. This buffer is often used in protein and DNA/RNA electrophoresis protocols, providing stable pH conditions that enhance separation and improve sensitivity during detection.
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661163.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L
TBE 10X Solution is a buffered solution commonly used in molecular biology and biochemistry. This 1-liter bottle contains a highly concentrated solution of Tris, borate, and EDTA (ethylene diamine tetraacetic acid), which is used as a running buffer for agarose gel electrophoresis. TBE helps maintain the pH and ionic strength of the electrophoresis buffer system, which is critical for the proper migration of nucleic acids. This 10X concentrated solution can be diluted to 1X for use in standard gel electrophoresis procedures. It is an essential reagent for DNA analysis, particularly in applications involving high-resolution separation of nucleic acid fragments.
Selleck Chemical LLC Trometamol hydrochloride S5300-100mg
Trometamol (THAM Tromethamine TRIS-HCL) hydrochloride a proton acceptor is a biologically inert amino alcohol of low toxicity which buffers carbon dioxide and acids in vitro and in vivo Trometamol rapidly restores pH and acid-base regulation in acidaemia
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660682.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L
TBE 10X Solution is an essential buffer used in the electrophoresis of nucleic acids such as DNA and RNA. This 4-liter bottle contains a concentrated mixture of Tris, borate, and EDTA, which provides the necessary pH and ionic environment for the proper migration of nucleic acid samples. TBE is particularly effective for separating small DNA fragments and is widely used in both research and clinical laboratories. By diluting the 10X stock to 1X, laboratories can prepare a buffer suitable for use in standard electrophoresis procedures. It is an important reagent for molecular biology applications requiring high-purity separation of nucleic acids.