Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005313 TRIS HYDROCHLORIDE 100G
SAPPHIRE NORTH AMERICA ALUMINIUM CRYO TBS + CPS 15ML
Aluminium cryo tubes + caps, 15 ml, 29mmx 30mm / 100 pcs
Bioworld TTETRIS-TAPS-EDTA) BUFFER
This item has a minimum order quantity of 2 per supplier requirements. TTE (TRIS-TAPS-EDTA) BUFFER POWDER BLEND: - Proteomics Grade - An electrophoresis running buffer for DNA Sequencing that yields longer reads
Dojindo Molecular Technologies Inc EPPS ( 25G), Good's buffer, CAS: 16052-06-5, pka=8.00, Active pH 7.5-8.5, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Sigma Aldrich Fine Chemicals Biosciences TRIS BUFFERED SALINE, WITH BSA
Tris Buffered Saline, with BSA, pH 8.0 Powder
eMolecules 1262005-64-0 | 5-Chloro-3-(4-hydroxymethylphenyl)benzoic acid | Combi-Blocks | MFCD18319466 | 262.690 | C14H11ClO3 | 97.000 | OCc1ccc(cc1)-c1cc(Cl)cc(c1)C(O)=O | 1g | 117590082
5-Chloro-3-(4-hydroxymethylphenyl)benzoic acid | Combi-Blocks | 1262005-64-0 | MFCD18319466 | 262.690 | C14H11ClO3 | 97.000 | OCc1ccc(cc1)-c1cc(Cl)cc(c1)C(O)=O | 1g | 117590082
Biotang Inc RNA Sample Loading Buffer (No Eth Bromide), 1 ml
RNA Sample Loading Buffer (No Eth Bromide), 1 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml.
Chemscene ChemScene | Methyl 6-chloro-5-(hydroxymethyl)picolinate | 250MG | CS-0197753 | 0.98 | 1205671-72-2| MFCD29035246 | 201.61
ChemScene | Methyl 6-chloro-5-(hydroxymethyl)picolinate | 250MG | CS-0197753 | 0.98 | 1205671-72-2| MFCD29035246 | 201.61