Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000414468-logo.png-250.jpg){kind=logo}
Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G
TAPSO is a common biological buffer and pH stabilization reagent. It has an effective pH ranging from 7-8 and pKa values ranging from 7.5-9.0. This compound contains Tris groups and exhibits reactive activity with zwitterionic glycine peptides.
- Common biological buffer
- pH stabilization reagent
- Effective pH ranging from 7-8
- pKa values ranging from 7.5-9.0
- Contains Tris groups
- Reactive with zwitterionic glycine peptides
- Suitable for research applications
Sigma Aldrich Fine Chemicals Biosciences PIPES BioXtra 99 titration250G
PIPES is a member of the ethanesulfonic acid buffer series first introduced by Good et al. developed to meet certain criteria midrange pKa maximum water solubility and minimum solubility in all other solvents minimal salt effects minimal change in pKa with temperature chemically and enzymatically stable minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 C is near physiological pH PIPES has applications in cell culture work.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
A measured mixture of chemically defined ingredients makes up a basal medium which is a type of complex media. DMEM is a medium supplemented with serum and sometimes with tryptose phosphate broth (TPB). DMEM/f-12 is a completely defined synthetic medium which is protein-free.
Sigma Aldrich Fine Chemicals Biosciences MOPS sodium salt | 71119-22-7 | MFCD00064350 | 100 g
MOPS sodium salt | Purity: ≥99.5% | Mol Wt: 231.25 | 71119-22-7 | MFCD00064350 | 100 g
Sigma Aldrich Fine Chemicals Biosciences HEPES Sodium Salt | 75277-39-3 | MFCD00036463 | 100g
HEPES Sodium Salt | Purity: 99% | Mol Wt: 260.29 | 75277-39-3 | MFCD00036463 | 100g
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt | 71119-23-8 | MFCD00065473 | 100g
MES sodium salt | Purity: 99% | MW: 217.22 | 71119-23-8 | MFCD00065473 | 100g
Biotang Inc TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml
TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml.
Medchemexpress LLC HEPES hemisodium salt | 103404-87-1 | MFCD00036463 | 99.0% | 249.80 | C8H18N2O4S·1/2Na | 100 MG
HEPES hemisodium salt is a zwitterionic buffering reagent commonly used to maintain physiological pH in biochemical assays and cell-culture media. Supplied as a solid powder, it is intended for preparation of HEPES buffer solutions and includes storage and handling guidance for laboratory use.
- High buffering capacity at physiological pH (6.8-8.2).
- Available as a solid powder for convenient solution preparation.
- Reported high purity suitable for analytical and cell-culture applications.
- Stable with sealed storage at 4°C and protected from moisture.
- Provided with supporting documentation (datasheet and safety information).