Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 20X PBS Solution, pH 7.2 (at 1X). 10L, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
Suitable for growth of a wide range of cell types in low serum conditions. The removal of phenol red is often preferred by investigators working with clonal and stem cell cultures.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
![Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661398.jpg-250.jpg)
Alkali Scientific PBS [Phosphate Buffered Saline] 200mL Tablets, 100 Tablets per Bottle
PBS [Phosphate Buffered Saline] 200mL Tablets are solid tablets designed for easy dissolution in water, making it simple to prepare 200mL of phosphate-buffered saline. These tablets are ideal for laboratories that frequently need to prepare large volumes of PBS for use in cell culture, microbiology, and molecular biology research. The tablets dissolve quickly and efficiently, ensuring that the resulting solution maintains the proper pH and osmolarity for reliable experimental results. Each bottle contains 100 tablets, providing an economical and convenient way to prepare PBS without the need for precise measuring or mixing of individual components.
Apexbio Technology LLC Annexin V-APC Reagent 20tests
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds phosphatidylserine (PS) Under normal conditions PS is located on the inner side of the plasma membrane lipid bilayer however during early apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane becoming exposed to the extracellular environment Annexin V has a high affinity for PS Fluorescent (APC)-labeled Annexin V serves as a fluorescent probe enabling detection of apoptotic cells using flow cytometry or fluorescence microscopy The staining procedure takes only 15-30 minutes to complete Use of this reagent requires additional purchase of 10X Binding Buffer (Catalog no K2284) To further characterize different apoptosis stages Annexin V can be combined with nuclear dyes such as 7-AAD DAPI PI or SYTOX Green These nuclear dyes specifically bind double-stranded DNA generating fluorescence Normally they cannot penetrate intac
Teknova 1X PBS with 200mM Sodium Chlor
1X PBS with 200mM Sodium Chloride, 0.001% P-188, 1000mL, Sterile 8 pack.
Sigma Aldrich Fine Chemicals Biosciences Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Tris Buffered Saline BioUltra, tablet (for 500 mL), pH 7.6 | MFCD00132476 | 10TAB
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This modification has been shown to improve the stability of the medium.
