Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences MES Monohydrate | 145224-94-8 | MFCD00149409 | 100g
MES Monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 100g
Discovery Life Sciences .5M POTASSSIUM PHOS BUFFER 7.4
Potassium Phosphate Buffer, P450 Enzyme Buffer, 0.5M
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate | 145224-94-8 | MFCD00149409 | 500g
MES monohydrate | Purity: 99% | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 500g
Sigma Aldrich Fine Chemicals Biosciences MES monohydrate BioUltra, for molecular biology, >=99.5% (T) | 145224-94-8 | MFCD00149409 | 50G
MES monohydrate BioUltra, for molecular biology, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 213.25 | 145224-94-8 | MFCD00149409 | 50G
Research Products International Corp PBS, 10X Powder Concentrate, 10 L
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.
Cell Signaling Technology Tris-Glycine SDS Running Buffer (10X) 1000 ml
Tris-Glycine SDS Running Buffer (10X) 1000 ml
Research Products International Corp RPI TRIS-CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
This product is shipped with one 500 ml Anode bottle and one 500 ml Cathode bottle
10X Anode Formulation:
TRIS: 0.6 M
CAPS: 0.4M
10X Cathode Formulation:
TRIS: 0.6 M
CAPS: 0.4 M
SDS: 1%
Research Products International Corp TAPS, 100 G
pKa(25°:C): 8.4. The working pH range in aqueous solution is 7.7-9.1.
![Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P10400-500.0.JPG-250.jpg)
Research Products International Corp PBS 1X Solution [Phosphate Buffered Saline 1X Sterile Solution], 500 Milliliters
PBS 1X Solution (Phosphate-Buffered Saline) is a balanced, isotonic buffer commonly used in biological and biomedical research. Prepared at a working concentration, it contains sodium chloride, sodium phosphate, and potassium phosphate to maintain pH and osmolarity. It is widely used in cell culture, molecular biology, and immunohistochemistry for cell washing, reagent preparation, and sample dilution. PBS helps preserve the integrity and viability of biological samples by providing a stable, non-disruptive environment. Its compatibility with most biological systems makes it a fundamental laboratory reagent.
Research Products International Corp NP-40 Lysis High Salt Buffer Solution, 500 ML
1X buffer used for extracting proteins from cells.
Research Products International Corp TRIS-Tricine-SDS Running Buffer 10X Solution, 500 ML
10X Formulation:
Tricine: 1 M
SDS (pH 8.3): 1%