Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml
TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml.
Medchemexpress LLC PBS-T Powder | 100 POUCHES
MCE PBS-T Powder (1 L of 1x) is a formulation consisting of NaCl, KCl, Phosphate, and Tween 20. It is extensively utilized as a washing buffer in immunoassay experiments such as ELISA and Western Blot. Additionally, it can be employed for antibody dilution and the preparation of blocking solutions. The product is conveniently packaged, with 20 pouches defined as the base specification, and larger sizes offered as incremental volumes of this base. Each pouch is designed to be diluted to 1 L of 1x PBS-T buffer.
- Widely used in ELISA and Western Blot.
- Can be used for antibody dilution and preparation of blocking solution.
- Consists of NaCl, KCl, Phosphate, and Tween 20.
- Each pouch dilutes to 1 L of 1x PBS-T buffer.
- Eliminates the need to weigh and mix individual components.
- Convenient way to make PBS-T buffer.
- Final 1x PBS-T buffer has a pH of 7.4 ± 0.2 @ 25°C.
Bioworld Tris-HCl Buffer 10mM with 50mM NaCl, pH 8.0, 250 mL
Tris-HCl Buffer has a 10mM concentration combined with 50mM NaCl and pH of 8.0. Tris-HCl Buffer with NaCl is a common biological buffer with many applications in molecular biology.
GenDEPOT TBST(10X), pH 7.4 | 1L
TBST(10X) is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays. The 10X TBST (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.14M NaCl, 0.1% Tween-20, pH 7.4, when diluted to 1X with water.
Boston Bioproducts Inc Bovine Serum Albumin, BSA (30% in DPBS) - 4x50ml
Bovine Serum Albumin BSA (30% in DPBS) - 4x50ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc TAPS Buffer (1 M, pH 8.5) - 100ml
TAPS Buffer (1 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4, RNase Free) - 250ml
Tris-HCl Buffer (1 M pH 7 4 RNase Free) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
Research Products International Corp MES, Anhydrous, 1 Kilograms
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp MES, Anhydrous, 25 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
![Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92040-5000.0.JPG-250.jpg)
Research Products International Corp MOPS, Sodium Salt [3-(N-Morpholino)-propanesulfonic acid sodium salt], 5 Kilograms
MOPS Sodium Salt, also known as 3-(N-Morpholino) propanesulfonic acid sodium salt, is a chemical compound that plays a significant role in the life science marketplace. It is a zwitterionic buffer commonly used in biological and biochemical research and is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures.
It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions. MOPS Sodium Salt is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
Research Products International Corp Potassium Phosphate, Dibasic, 3 Kilograms
Potassium Phosphate Dibasic, Anhydrous (dipotassium phosphate) is a buffering agent commonly used in molecular biology, biochemistry, and cell culture. It effectively maintains pH in the neutral to slightly alkaline range (7.0–8.0), making it ideal for applications such as protein purification, enzyme assays, and cell culture media. Its strong buffering capacity ensures experimental consistency. In addition to pH control, it serves as a potassium ion source—supporting cellular processes and metabolism. Its dual role as a pH stabilizer and nutrient makes it valuable in media formulation and biochemical research.
![Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P32200-1000.031.JPG-250.jpg)
Research Products International Corp PBS [Phosphate Buffered Saline], 10X Powder Concentrate, White Granular Powder, 1 Liter
PBS, 10X Powder Concentrate is a highly concentrated phosphate-buffered saline solution used in biology and biochemistry research. It contains sodium chloride, sodium phosphate, and potassium phosphate, which maintain pH and osmolarity. Designed for dilution before use, it offers a convenient and cost-effective way to prepare large volumes of 1X PBS. Once diluted, it serves as an isotonic buffer for cell culture, immunohistochemistry, protein work, and molecular biology. It supports cell and tissue integrity by maintaining stable physiological conditions during washing, staining, or sample preparation.