Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Fd Neurotechnologies Inc PHOSPH-BUF .04PARAFORM W/ PA
Phosphate-Buffered 0.04 Paraformaldehyde with Picric Acid
Teknova 20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 2 pack.
2M Tris,3M Sodium Chloride,1.0% Tween-20
Teknova 10X PBS Solution, 4L, Non-Sterile. 4 pack.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1M HEPES, pH 6.8. 1000mL, Sterile 2 pack.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 1M Tris-HCl, pH 7.5. 500mL, Sterile, DNAse/RNAse Tested 8 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 50X TAE, Electrophoresis Grade. (2M Tris-Acetate, 100mM EDTA) 1000mL, Non-Sterile. 2 pack.
2M Tris-Acetate, 100mM EDTA
eMolecules tert-butyl 4-(hydroxymethyl)-2,2-dimethyl-piperidine-1-carboxylate | 2167889-81-6 | MFCD30497054 | 1g
Pharmablock - Stock | tert-butyl 4-(hydroxymethyl)-2,2-dimethyl-piperidine-1-carboxylate | 1g | 521639997 | PBU1776 | | 2167889-81-6 | MFCD30497054 | 243.347 | C13H25NO3
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Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.4 (0.2 micron filtered), 500 ml.
Teknova 50X TAE Solution. 1000mL, Non-Sterile.
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova 1M HEPES Solution, pH 6.5. 1000 mL, Sterile.
HEPES solution is a Goods buffer that is commonly used in cell culture and biochemical applications. It is well known for its ability to maintain pH level, making it an essential part of any experiment that requires a stable pH. It has a buffering range of 6.8 - 8.2.
Teknova 0.5M MES Buffer, pH 6.1. 500mL, Sterile.
MES solution is a zwitterionic buffer used to help maintain pH levels in biochemistry experiments. It has a buffering range of pH 5.8 - 6.5.
Teknova 0.9% Saline Solution. 1000mL, Sterile. Endotoxin Tested.
Sodium chloride is an essential ingredient in the lab. Not only does it help regulate the pH of solutions, but it also prevents growth of bacteria and other contaminants. In addition, salt is a powerful desiccant, meaning that it can help to dry out wet samples. As a result, salt is often used in a wide variety of laboratory procedures.
Teknova 0.5M Tris, 1.5M Sodium Chloride, 0.5% Tween-20, pH 7.7. 500mL, Sterile.
0.5M Tris,1.5M Sodium Chloride,0.5% Tween-20
Teknova 1X PBS with 0.1% Tween 80. 1000mL. Sterile.
137.0mM Sodium Chloride, 4.3mM Sodium Phosphate Dibasic, 2.7mM Potassium Chloride, 1.4mM Potassium Phosphate Monobasic,0.1% Tween 80