Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotium Fixation Permeabilization Other Buffers DNA Gel Stains and Accessories DNA Gel Stains Chemical Reagents 50/PK
TBE (Tris-borate-EDTA) buffer powder packet for preparing 1 L of 1X TBE buffer for nucleic acid gel electrophoresis Box includes 50 packets
MSC Value Collection Magnifier, 20x, Dual Lens, Round, Metal
The Value Collection magnifier is a durable tool designed for detailed inspection. It features a high 20x magnification, a dual-lens system, and a robust metal construction, making it suitable for various precision tasks.
- 20x maximum magnification
- Dual lens system
- Round lens shape
- Metal construction
CARTERRA INC 0.1 M MES PH 5.5
NC2232564 0.1 M MES PH 5.5
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 1L
Tris-HCl Buffer (1 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Hampton Research HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml
HR2-733/1.0 M HEPES sodium pH 7.5 - 100 ml. Please note items from this supplier are non-returnable.
Electron Microscopy Sciences R-Universal Epitope Recovery Buffer (10x stock) 500 ML
EMS UNIVERSAL Epitope Recovery Buffer is a novel (patent-pending) technology for epitope recovery, primarily based on reversing the fixation effects of formaldehyde, which created links primarily between ε-amino groups of lysine and other amino-groups. In extensive testing the buffer has shown excellent results when used with different antibodies, including those that require low or high pH buffers or protolithic treatment for successful staining.
The EMS Universal Buffer may be used in any heat-induced epitope recovery system (the time and temperature of treatment should be tested), but was specifically adjusted for tissue sections processing in our Antigen Retriever (62700 Series). Using our Universal Buffer in our Retriever guarantees the highest rate of success in recovering epitope for any antibody, especially one that was never previously used on formalin-fixed sections. Shelf Life: 12 months.
Quality Biological Inc TBS (10X), pH 7.4, 4 Liters
TBS (10X), pH 7.4 is used as a washing buffer for cellular and molecular biology techniques.
DNase, RNase and Protease tested.
Applications: TBS (10X), pH 7.4 will be useful as a washing buffer for electrodes, microtiter plates, and alkaline phophatase or peroxidase conjugates. TBS (10X), pH 7.44 can also be used in detergent preparation and Western blot or ELISA applications.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase, and Protease Tested
pH: 7.4 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Quality Biological Inc PBS (10X), pH 7.4, 4 Liters
PBS is a balanced salt solution used for washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Useful in immunological applications, such as Western Blotting.
Without Calcium and Magnesium
Endotoxin and Mycoplasma tested
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
Osmolality: 300 ± 55 mOsm/Kg H20
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Sigma Aldrich Fine Chemicals Biosciences MOPSO sodium salt 99 titra500G
MOPSO sodium salt 99 titra500G