Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Crescent Chemical Co Inc SERVA TRIS-GLYCINE/SDS BUFFER
SERVA Tris-Glycine/SDS sample buffer (2x) Sample buffer for SDS PAGE. Supplied as 2 x concentrate. Contains 126 mM Tris/HCl (pH 6.8), 20 % glycerol, 4 % SDS and 0.02 % bromophenol blue. Size - 20ML Storage Conditions - +2 °C TO +8 °C Catalog Number - 42527.01
Med Vet International Irrigation Solution Saljet 0.9% Sodium Chloride, Preservative Free Not for Injection Unit Dose Vial 30 mL, 64938-009-01, 240/Case
0.9% sodium chloride weight by volume Each 30-mL vial has a twist-off top 40 vials per box
Carterra 10x HBS no Tween - 1 Liter
10x HBS (no Tween) - 1 Liter
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.8 (0.2 micron filtered), 500 ml.
Thermo Scientific Production Chemicals and Services TRIS HCL GMP GRADE 50KG
NC3654763 TRIS HCL GMP GRADE 50KG
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.0 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.0 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml
TRIS Buffered Saline, (20X) Tris 500 mM KCL 54 mM NaCl 2.74M, 1000 ml.
Biotang Inc Citrate-buffered Saline or SSC (20X), 1000 ml
Citrate-buffered Saline or SSC (20X), 1000 ml.
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660682.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L
TBE 10X Solution is an essential buffer used in the electrophoresis of nucleic acids such as DNA and RNA. This 4-liter bottle contains a concentrated mixture of Tris, borate, and EDTA, which provides the necessary pH and ionic environment for the proper migration of nucleic acid samples. TBE is particularly effective for separating small DNA fragments and is widely used in both research and clinical laboratories. By diluting the 10X stock to 1X, laboratories can prepare a buffer suitable for use in standard electrophoresis procedures. It is an important reagent for molecular biology applications requiring high-purity separation of nucleic acids.