Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Teknova 20X TBS with 1% Tween-20, pH 7.5. (2M Tris, 3M NaCl) 1000mL, Sterile. 2 pack.
2M Tris,3M Sodium Chloride,1.0% Tween-20
Andwin Scientific VWR PBS-T BUFFER POWDER 100/CS
VWR® Buffer Powder Packs can save time and money, as there is no need to measure and weight many separate chemical components which is required when making buffers from scratch.Buffer powder quickly dissolves to make 1L of 1X solutionNo weighing or measuring of separate componentsNo pH adjustments requiredEconomical shipping, long shelf lifeQuality and consistency guaranteedVWR® Pre-measured buffer packs are extremely easy to use and are formulated so each pack makes 1 Liter of 1X buffer. Simply tear open a pack and mix completely with 1 liter of distilled water.Shipping costs are also minimal, as each case of 50 or 100 packs is compact and weighs only 2.5 Lbs.
AdipoGen PBS Endotoxin-free (sterile) (3 x 10 ml)
PBS Endotoxin-free (sterile) (3 x 10 ml), Adipogen Life Sciences. Chemical. Endotoxin free buffer used for the dilution of TLRpure LPS and solubilisation of endotoxin-free reagents (e.g. ODNs).
![Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-P40140-5000.0.JPG-250.jpg)
Research Products International Corp RPI PIPES [Piperazine-N-N'-bis(2-ethanesulfonic acid)], 5 Kilograms
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Boston Bioproducts Inc Paraformaldehyde (4%, PBS with Mg & EGTA) - 250ml
Paraformaldehyde (4% PBS with Mg and EGTA) - 250ml (For Research Use and Further Manufacturing Only)
Alkali Scientific CellPro™ DPBS 10X, 500ml, 10/Case
Balanced salt solution used for multiple cell culture applications, (liquid form). Certificate of Analysis available electronically. Standard stock items from 100 mL to 200 L Volumes up to 1000 L available as made to order Not for diagnostic or therapeutic use. For making isotonic solutions for cell or tissue rinsing, cell dilution, reagent prep, transportation etc. where maintenance of cell integrity is required for further manufacturing or research use.
Biotang Inc RNAse Free Phosphate-Buffered Saline, 250 ml
RNAse Free Phosphate-Buffered Saline, 250 ml.
Revvity Health Sciences Inc DELFIA L*R wash concentrate (25x) Standard, 250 mL
DELFIA L*R wash concentrate (25x) Standard, 250 mL