Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp PBS 1X SOLN 1ML TUBES 36/CS
Ready to use sterile 1X solution, supplied in sterile pre-filled screw cap tubes. Phosphate Buffered Saline is a buffer solution commonly used in biological research. PBS has many uses because it is isotonic and non-toxic to most cells. Each tube contains 1.0 ml PBS 1X Sterile Solution, packaged in a sealed white cardboard freezer box with a 6 x 6 divider. PBS is supplied in 4.5 ml screw cap tubes that are 3.5 “ high (cap on). Each case holds 36 pre-filled tubes.
Convenient 1.0 ml of PBS per tube
Sterile
36 x 1.0 ml
Biotang Inc TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
Boston Bioproducts Inc MOPS Buffer (1 M, pH 7.0) - 500ml
The MOPS Buffer [3- (N-morpholino)propanesulfonic acid buffer] is a versatile zwitterionic Good's buffer used in biological research. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH, and maintains a pH range from 6.5 to 7.9. It is often used as a buffering agent in biology and biochemistry, and is recommended for polyacrylamide gel electrophoresis, as a running buffer, in separating RNA in agarose gels, and for protein purification chromatography.
IBI SCIENTIFIC 3P 1X PBST (PBS W/0.05% TWEEN 20) 6/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.05% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 1X PBST (1X PBS) with TWEEN 20 (0.05% TWEEN 20) is a ready-to-use sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 1X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
IBI SCIENTIFIC 3P 1X PBST (PBS W/0.05% TWEEN 20) 4/PACK
Sterile buffer solution containing sodium phosphate, sodium chloride, potassium phosphate and potassium chloride with 0.05% TWEEN 20.Sterile filtered using 0.1um filterDNase, RNase and protease free.Sterility validated using USP <71> Test for SterilityDoes not contain calcium, magnesium, phenol red or sodium pyruvate. IBI 1X PBST (1X PBS) with TWEEN 20 (0.05% TWEEN 20) is a ready-to-use sterile solution in a 1 liter bottle that is ideal for use in immunoassay procedures such as Western Blotting and ELISAs in microtiter plate wells.IBI 1X PBST is mixed and bottled in our liquid production facility here in Dubuque, Iowa. Our high output pure water system produces endotoxin free and nuclease free water at a rate of 100 gallons per/hr. This water is the basis for all of our liquid blending production here at IBI.
Research Products International Corp HEPES, Free Acid, 25 KG
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
IBI SCIENTIFIC 3P (EDTA) SOLUTION, 100 mL
IBI 0.5MEDTA solution (pH 8.0)is ideal for biochemistry and molecular biology applications requiring a chelator of divalent metal ions. Ethylenediamine tetraacetic acid solution can be used to elucidate the role of antigen retrieval in immunostaining of ethanol fixed smears.
Boston Bioproducts Inc Paraformaldehyde (4%, PBS with Mg & EGTA) - 250ml
Paraformaldehyde (4% PBS with Mg and EGTA) - 250ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (2X) - 500ml
HEPES Buffered Saline (2X) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Potassium Phosphate Buffer (0.2 M, pH 7.4) - 1L
Potassium Phosphate Buffer (0 2 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc 50 mM Sodium Phosphate, 2 mM EDTA (pH 7.0) - 2x1L
Bioprocess Grade - 50 mM Sodium Phosphate 2 mM EDTA (pH 7 0) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, for Western Blot Washing) - 1L
Phosphate Buffered Saline PBS (10X for Western Blot Washing) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Buffered Saline-Tween, TBST - 2x1L
Bioprocess Grade - Tris-Buffered Saline-Tween TBST - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 1L
Tris-HCl Buffer (1 M pH 8 0) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Phosphate Buffer (1 M, pH 7.2) - 50ml
Sodium Phosphate Buffer (1 M pH 7 2) - 50ml (For Research Use and Further Manufacturing Only)