Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC Annexin V-HF555/SYTOX Green Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but during early apoptosis PS is translocated from the inner leaflet to the outer surface of the cell membrane exposed to the extracellular environment Annexin V has a high affinity for PS and when labeled with fluorophore (HF555) Annexin V can serve as a fluorescent probe to detect apoptosis via flow cytometry or fluorescence microscopy The staining process requires only 10-20 minutes SYTOX Green specifically binds to double-stranded DNA emitting green fluorescence and normally cannot cross intact cell membranes However in late-stage apoptotic cells or dead cells SYTOX Green can penetrate the cell membrane and stain the nucleus By combining Annexin V-HF555 and SYTOX Green staining it is possible to distinguish among early apoptotic late apoptotic and dead cells
FUJIFILM Biosciences Alpha MEM Earles Salts w/o Nuc
Alpha MEM Earles Salts w/o Nucleosides is Modified Eagles Minimum Essential Medium designed for growth of a mouse-hamster-hybridoma cellLine 8-A
Hampton Research HR2-577/1.0 M HEPES sodium - 100 ml
HR2-577/1.0 M HEPES sodium - 100 ml. Please note items from this supplier are non-returnable.
Alkali Scientific MOPSO Sodium Salt, 2 Kg
MOPSO Sodium Salt is a buffering reagent used to stabilize pH in laboratory applications. The 2 kg weight offers ample supply for large-scale experiments, ensuring consistency and reproducibility in analytical procedures. This sodium salt of MOPSO (Morpholinopropanesulfonic acid) provides excellent buffering capacity, especially in the pH range of 7.0–8.5, which is critical for maintaining the integrity of sensitive biological samples and chemical reactions. MOPSO is frequently used in the preparation of buffers for chromatography, electrophoresis, and enzyme assays. Its ability to maintain pH stability in various solutions makes it an essential reagent for biochemical and pharmaceutical applications.
Biotang Inc HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545
HEPES Buffered Saline 5X, pH 6.5. 125 mM HEPES, 750 mM Sodium Chloride, pH 6.5. Prepared in 18.2 megohms water and filtered through 0.22 micron filter. BB-545.
Medchemexpress LLC AR-13324 analog mesylate | 2095432-73-6 | 98.0% | 631.72 | 5 MG
AR-13324 analog mesylate is an analog of AR-13324. This product is for research use only.
- Acts as a Rho-associated protein kinase (ROCK) inhibitor.
- Functions as a norepinephrine transporter (NET) inhibitor.
Bioworld ACES Buffer, 0.5M, pH 7.5, 250 mL
Product DescriptionACES (N-(2-acetamindo)-2-aminoethancesulfonic acid) buffer has a concentration of 0.5M and a pH level of 7.5. Buffer was prepared in 18.2 megohms water and filtered through 0.22 micron filter. ACES has been used to develop buffers for both agarose and polyacrylamide gel electrophoresis. A saturated solution contains 0.22M at 0°C and a melting point greater than 220°C. ACES binds Cu2+, but not significantly Mg2+, Ca2+, or Mn2+. Product is stored at room temperature.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659807.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 250 G
HEPES, Free Acid is a biochemical reagent used for maintaining a stable pH environment in biological and chemical applications. This 250g package is ideal for preparing buffered solutions for use in cell culture, protein analysis, and other biochemical experiments. HEPES, or 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, is widely used because of its excellent buffering capacity in the pH range of 6.8 to 8.2. It is commonly used in cell biology to maintain physiological pH during experiments. This reagent is highly stable, non-toxic to cells, and is ideal for applications that require precise pH control over extended periods of time.
![Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659860.jpg-250.jpg)
Alkali Scientific BES [N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid], 25 G
BES (N,N bis(2 Hydroxyethyl) 2 aminoethanesulfonic acid) is a biochemical reagent used as a buffering agent in laboratory applications. The 25g quantity is ideal for smaller experiments or laboratories with limited storage space. BES is known for its effective pH buffering capabilities, making it an essential component in protocols that require precise pH control, such as protein purification, electrophoresis, and enzyme assays. It helps maintain the stability of biochemical reactions and is frequently used in molecular biology, biochemistry, and medical research where consistent pH levels are critical for experimental success. The small size is ideal for smaller-scale experiments or specialized applications.