Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Aniara Diagnostica LLC PBS TBLT PH 7.4 1000ML 100 EA
Medicago Phosphate Buffered Saline (PBS) pH 7.4 supplied as preweighed tablets yields 1000 mL 0.14 M NaCl, 0.0027 M KCl, 0.010 M phosphate buffer with pH 7.4 at 25C. Use as biological buffer, isotonic. Bottle of 100 tablets.
Aniara Diagnostica LLC Phosphate Buffered Saline with Tween 20, pH 7.4, 100 tablets
Medicago Phosphate Buffered Saline w/Tween (PBS-T) pH 7.4 supplied as preweighed tablets yields 1000 mL 0.14 M NaCl, 0.0027 M KCl, 0.05% Tween-20, 0.01 M phosphate buffer with pH 7.4 at 25C. Use for immunoassays. Bottle of 100 Tabelts.
Kd Medical Inc PBS BUFFER, 1X, PH 7.2
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
Kd Medical Inc STERILE SALINE 0.9%, 100ML BTL
Solution used for sterile irrigation, rinsing, dilution and cell washing purposes. One 100ml bottle.
Kd Medical Inc PBS BUFFER, 1X, PH 7.2, 500ML
1X PBS working solution commonly used in biological research. Used as a wash buffer in several research applications. One 500ml bottle.
Kd Medical Inc TAE 10X 1L BOTTLE
TAE Buffer (Tris Actetate EDTA) is the most commonly used buffer for agarose DNA electrophoresis. 10X concentrated ready to be diluted to a 1X working solution. One 1 liter bottle.
Growcells TAE BUFFER 50X 1L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
Teknova DNA Suspension Buffer, pH 8.0. RNase/DNase Tested, PCR Grade, 1L, Sterile. (10mM Tris, 0.1mM EDTA)
TE buffer is a salt solution that is commonly used to resuspend (or dilute) DNA and RNA. Composed of Tris base and EDTA, these molecules work together to maintain pH and prevent nucleic acids from degradation.
Teknova 1M Tris-HCl, pH 7.0, 500ml, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 10X PBS with 0.5% Tween 20. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova 1X PBS with 0.05% Tween 20, pH 7.4. 1000mL, Sterile.
Phosphate-buffered saline (PBS) is a salt solution that is commonly used in physiological and biochemical experiments. It can be used to maintain pH neutrality or to buffer against changes in pH in order to provide optimal conditions for cellular growth and function. The buffering range is between pH 6.8 and 7.4.
Teknova PPB Solution. 1000mL, Sterile.
30mM Tris-HCl pH 8.0, 1mM Ethylenediaminetetraacetic acid (EDTA) pH 8.0,20% Sucrose
Teknova 1M Tris-HCl, pH 7.2 Solution. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Alkali Scientific CellPro™ PBS Buffer, 1X, pH 7.4, without Calcium or Magnesium, Sterile Filtered, 1000mL, 10/Case
CellPro™ PBS Buffer, 1X, pH 7.4, 1000mL Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.