Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Hepes|7365-45-9 | MFCD00006158 | 100g
Hepes | Purity: 99.5% (titration) | MW: 238.3 | 7365-45-9 | MFCD00006158 | 100g
Sigma Aldrich Fine Chemicals Biosciences HEPES | 7365-45-9 | MFCD00006158 | 250 g
HEPES | Purity: ≥99.5% | Mol Wt: 238.30 | 7365-45-9 | MFCD00006158 | 250 g
Sigma Aldrich Fine Chemicals Biosciences HEPES solution | 7365-45-9 | MFCD00006158 | 500ml
HEPES solution | Mol Wt: 238.306 | 7365-45-9 | MFCD00006158 | 500ml
Bio Rad Laboratories XT MOPS RUNNING BUFFER 500ML
Extended range protein standard, 500ul, 50 applications. Product contains 12 bands from 2kD to 250kD. 250, 150, 100, 50, 37, 20, 15, 10 & 5 kD are blue, 75, 25 and 2kD are pink.
Quality Biological Inc Tris (1 M) pH 9.8 1000mL
Suitable for molecular biology protein chemistry and biochemical applications.DNase RNase and Protease tested.Filtered through a 0.22 micron membraneDnase Rnase and Protease tested Manufactured in an ISO 90012015 certified facility.
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
![Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000414468-logo.png-250.jpg){kind=logo}
Medchemexpress LLC 1-Propanesulfonic acid, 2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]- | 68399-81-5 | 98.0% | 25 G
TAPSO is a common biological buffer and pH stabilization reagent. It has an effective pH ranging from 7-8 and pKa values ranging from 7.5-9.0. This compound contains Tris groups and exhibits reactive activity with zwitterionic glycine peptides.
- Common biological buffer
- pH stabilization reagent
- Effective pH ranging from 7-8
- pKa values ranging from 7.5-9.0
- Contains Tris groups
- Reactive with zwitterionic glycine peptides
- Suitable for research applications
Ethos Biosciences Buffer tris multipurpose classic 1/EA
Dilurex 1M Tris-HCL 10X Buffer is a classic laboratory buffer at pH 7 4 It does not include a saline ionic component and is free to be mixed with other components as needed in your laboratory kip the tedium of weighing powders and stocking raw materials that can expire Our buffer is convenient ready to use and will save you hours in the lab letting you focus on breakthroughs rather than buffer prep Thousands of researchers trust Ethos Biosciences buffers for their proven track record in top-tier publications and protocols - upgrade your productivity today
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G