Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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361 – 375 of 3,492 results

361

TBS-TWEEN™ Tablets, MilliporeSigma™

Convenient, pre-measured tablet format for preparing Tris-buffered saline with 0.05% TWEEN™ 20 detergent.

Pricing & Availability

362

MilliporeSigma™ TBS Tablets, Calbiochem™,

Dissolving one tablet in 500mL of deionized H₂O yields 150mM NaCl, 50mM Tris-HCl buffer

Pricing & Availability

363

MilliporeSigma™ HEPES, Free Acid, OmniPur™, Calbiochem™,

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Synonym	N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

364

Promotions Available

Thermo Scientific Chemicals BICINE, 99%

CAS: 150-25-4 Molecular Formula: C6H12NNaO4 Molecular Weight (g/mol): 185.16 MDL Number: MFCD00004295 InChI Key: MFBDBXAVPLFMNJ-UHFFFAOYSA-M Synonym: N,N-Bis(2-hydroxyethyl)glycine PubChem CID: 8761 ChEBI: CHEBI:40957 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]acetic acid SMILES: [Na+].OCCN(CCO)CC([O-])=O

Pricing & Availability
Specifications

PubChem CID	8761
CAS	150-25-4
Molecular Weight (g/mol)	185.16
ChEBI	CHEBI:40957
MDL Number	MFCD00004295
SMILES	[Na+].OCCN(CCO)CC([O-])=O
Synonym	N,N-Bis(2-hydroxyethyl)glycine
IUPAC Name	2-[bis(2-hydroxyethyl)amino]acetic acid
InChI Key	MFBDBXAVPLFMNJ-UHFFFAOYSA-M
Molecular Formula	C6H12NNaO4

365

MilliporeSigma™ Calbiochem™ OmniPur™ 100X TE Buffer Solution Liquid Concentrate

For use in molecular biology applications

Pricing & Availability

366

TBE 10X Buffer, MP Biomedicals™

MDL Number: MFCD00236358 Synonym: Tris-Borate-EDTA buffer

Pricing & Availability
Specifications

MDL Number	MFCD00236358
Synonym	Tris-Borate-EDTA buffer

367

MilliporeSigma™ Calbiochem™ OmniPur™ PBS 10X Liquid Concentrate, Molecular Biology Grade

Makes 10L of 1X PBS Buffer from each liter.

Pricing & Availability
Specifications

Chemical Name or Material	Phosphate Buffered Saline (PBS)
Recommended Storage	25°C

368

Thermo Scientific Chemicals TE buffer, (20X), pH 8.0, autoclaved

CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 MDL Number: MFCD00236359 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl

Pricing & Availability
Specifications

PubChem CID	93573
CAS	1185-53-1
Molecular Weight (g/mol)	157.594
MDL Number	MFCD00236359
SMILES	C(C(CO)(CO)N)O.Cl
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride
InChI Key	QKNYBSVHEMOAJP-UHFFFAOYSA-N
Molecular Formula	C4H12ClNO3

369

MilliporeSigma™ Calbiochem™ OmniPur™ PBS 10X Liquid Concentrate, Molecular Biology Grade, Sterile

Makes 10L of 1X PBS Buffer from each liter.

Pricing & Availability

370

Thermo Scientific Chemicals AMPSO, 0.2M buffer soln., pH 9.0

CAS: 102029-60-7 Molecular Formula: C7H16NNaO5S Molecular Weight (g/mol): 249.257 MDL Number: MFCD00067500 InChI Key: UVGYCHPTWHWEPZ-UHFFFAOYSA-M PubChem CID: 44119996 IUPAC Name: sodium;2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonate SMILES: CC(C)(CO)NCC(CS(=O)(=O)[O-])O.[Na+]

Pricing & Availability
Specifications

PubChem CID	44119996
CAS	102029-60-7
Molecular Weight (g/mol)	249.257
MDL Number	MFCD00067500
SMILES	CC(C)(CO)NCC(CS(=O)(=O)[O-])O.[Na+]
IUPAC Name	sodium;2-hydroxy-3-[(1-hydroxy-2-methylpropan-2-yl)amino]propane-1-sulfonate
InChI Key	UVGYCHPTWHWEPZ-UHFFFAOYSA-M
Molecular Formula	C7H16NNaO5S

371

GTE buffer, Thermo Scientific™

For miniprep and DNA purification

Pricing & Availability
Specifications

Physical Form	Liquid
Chemical Name or Material	GTE buffer
TSCA	Yes
Recommended Storage	Keep cold

372

Thermo Scientific Chemicals HEPES, 1.0M buffer soln., pH 7.0

CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1

Pricing & Availability
Specifications

PubChem CID	23831
CAS	7365-45-9
Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
MDL Number	MFCD00006158
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
IUPAC Name	2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
Molecular Formula	C8H18N2O4S

373

Thermo Scientific Chemicals MOPS, 0.5M buffer soln., pH 7.5

CAS: 1132-61-2 Molecular Formula: C7H15NO4S Molecular Weight (g/mol): 209.26 MDL Number: MFCD00006183 InChI Key: DVLFYONBTKHTER-UHFFFAOYSA-N PubChem CID: 70807 ChEBI: CHEBI:44115 IUPAC Name: 3-morpholin-4-ylpropane-1-sulfonic acid SMILES: [O-]S(=O)(=O)CCC[NH+]1CCOCC1

Pricing & Availability
Specifications

PubChem CID	70807
CAS	1132-61-2
Molecular Weight (g/mol)	209.26
ChEBI	CHEBI:44115
MDL Number	MFCD00006183
SMILES	[O-]S(=O)(=O)CCC[NH+]1CCOCC1
IUPAC Name	3-morpholin-4-ylpropane-1-sulfonic acid
InChI Key	DVLFYONBTKHTER-UHFFFAOYSA-N
Molecular Formula	C7H15NO4S