Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc Tricine-buffered Saline (5X) pH 7.0, 250 ml
Tricine-buffered Saline (5X) pH 7.0, 250 ml.
Research Products International Corp RPI TRIS-CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
This product is shipped with one 500 ml Anode bottle and one 500 ml Cathode bottle
10X Anode Formulation:
TRIS: 0.6 M
CAPS: 0.4M
10X Cathode Formulation:
TRIS: 0.6 M
CAPS: 0.4 M
SDS: 1%
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 25g
Trizma base | Purity: 99.9% | MW: 121.14 | 77-86-1 | MFCD00004679 | 25g
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959187 ELUTE BUFFER A 50MM TRIS HCL8
Zymo Research Corporation DNA Wash Buffer (Concentrate), 48ml
This ensures high purity DNA to be recovered. Ethanol must be added to the concentrate prior to use. Instructions for each DNA Wash Buffer (concentrate) size is listed on the bottle and within the Zymo Research protocols it is used in.
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 10L Sto
eMolecules 143726-85-6 | tert-Butyl 4-(hydroxymethyl)benzoate | MFCD04973449 | 1g
Ambeed | 3-Aminopentanedioic acid | 100mg | 525093990 | A176948 | 1948-48-7 | MFCD00056698 | 147.130 | C5H9NO4
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Quality Biological Inc Tris-HCl (1M), pH 7.5, 1000 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. These solutions of Tris Hydrochloride provide convenient and highly purified solutions. Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).
Use Tris-HCl (1M), pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase, RNase and Protease Tested
pH Range: 7.5 ± 0.1
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.