Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioXtra, >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 500G
BIS-TRIS BioXtra, >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 500G
Sigma Aldrich Fine Chemicals Biosciences mPAGER 4-12 Bis-Tris Preca
mPAGE 4-12% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 1kg
BIS-TRIS | Purity: ≥98.0% (titration) | MW:209.24 | 6976-37-0 | MFCD00002853 | 1kg
Alkali Scientific Tris Acetate Ultra Pure, 500 G
Tris Acetate Ultra Pure is a high-quality buffering agent formulated for high-precision laboratory applications. With its ultra-pure grade, it ensures minimal interference in sensitive experiments, maintaining a stable pH environment. This 500g bottle is ideal for medium-scale preparations, supporting a wide range of biochemical and molecular biology applications, including electrophoresis, protein assays, and enzyme reactions. The tris acetate buffer is particularly effective for experiments requiring consistent pH conditions, making it a reliable choice for researchers working in protein analysis, DNA/RNA extraction, and various other scientific fields where precision is critical.
Growcells TAE BUFFER 10X 2L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 48.46g TRIS Base, 11.4ml Acetic Acid, 3.72g EDTA. pH 8.3
Bioworld ACE TBE w/ 7M Urea, pH 8.6, 250 mL
Product DescriptionA.C.E. TM TBE (Tris-Borate-EDTA) buffer comes with 7M of Urea with a pH level of 8.6. A.C.E. TM buffers deliver consistent and reproducible results because they are manufactured with the highest quality reagents in nuclease free water. Product is shipped at room temperature.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS BioXtra, >=98.0% (titration) | 6976-37-0 | MFCD00002853 | 25G
BIS-TRIS BioXtra, >=98.0% (titration) | Purity: >=98.0% (titration) | Mol Wt: 209.24 | 6976-37-0 | MFCD00002853 | 25G
Hampton Research HR2-579/1.0 M TRIS hydrochloride - 100 ml
HR2-579/1.0 M TRIS hydrochloride - 100 ml. Please note items from this supplier are non-returnable.
Bioworld STEBuffer (1X), 4 L
STE Reagent Common reagent for molecular biology. Used in DNA extraction, DNA electrophoresis and many other lab protocols.DNAse and RNAse free, ready to use 1X solution Technical Information:NaCl (100mM) Tris-HCl, pH 8.0 (10mM) EDTA, pH 8.0 (1mM)