Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld BES Buffer, 0.5M, pH 7.5, 100 mL
Product DescriptionBES (2-(Bis(2-hydroxyethyl) amino) ethanesulfonic acid) buffer has a concentration of 0.5M and a pH value of 7.5. BES is a zwitterionic buffer used in biochemistry and molecular biology, and has been used to study versatile catalyst precursors for mild hydrocarboxylation of alkanes to carboxylic acids. BES was used to determine that saline buffers minimize the action of interfering factors in the bacterial endotoxins test.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Bioworld Bis-Tris, 100 g
Synonym: 2,2-Bis(hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis(2-hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propanediol, Bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane A common biological bufferAn inert, zwitterionic buffer that exhibits only a very small shift in dissociation constantSpecifications:Ultra Pure GradepKa (25°C): 6.5+/-0.2Appearance: SolidBoiling Point : 466.18 °C at 760 mmHg (Predicted)Density : 1.35 g/cm3 (Predicted)Melting Point: 102-105 °CPH Value: 5.8-7.2
Sigma Aldrich Fine Chemicals Biosciences Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Tris-Borate-EDTA buffer | MFCD00236358 | 4 L
Research Products International Corp TAE, 10 L
TAE 10X Solution is a concentrated Tris-acetate-EDTA buffer used in molecular biology for DNA and RNA electrophoresis. When diluted to 1X, it maintains pH (~8.3) and low ionic strength. It supports clear nucleic acid separation, extended run times, and protects samples from degradation by chelating divalent metal ions like Mg²⁺.
Substance Name: [TRIS Acetate EDTA 10X Solution]
10X solution contains:
TRIS-Base: 0.4 M
Acetic Acid: 0.2 M
EDTA: 10 mM
pH (10X Solution (@25°C): 8.2-8.4
Research Products International Corp ADA 100 G
- Substance Name: [N-(Carbamoylmethyl) iminodiacetic acid)] [N-(2-Aceta-mido)iminodiacetic acid]
- CAS Number: 26239-55-4
- Molecular Formula: C6H10N2O5
- Molecular Weight: 190.2
Medchemexpress LLC TAE-1 25mg | 1414469-59-2 | 25 MG
TAE-1 is a small-molecule inhibitor of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) that also inhibits amyloid-β fibril formation and aggregation. It is used in biochemical and cellular research into Alzheimer's disease, cholinesterase function, and amyloid-β aggregation.
- Potent inhibitor of AChE and BuChE.
- Inhibits amyloid-β fibril formation and aggregation.
- Molecular formula C39H51I3N6O9 and molecular weight 1128.57.
- Typical supplied purity about 96.17%.
- Available in small laboratory pack sizes (for example, 25 mg) for research use.
Bioworld TTE Buffer, Ready-Pack (20X), 1 PK(s)
20x TTE (Tris-TAPS-EDTA) buffer pack can be dissolved into 2L of water to make 2L of 1x TTE solution.
Alkali Scientific CellPro™ PBS Buffer, 1X pH 7.2, 100mL
Alkali Scientific™ CellPro™ PBS (Phosphate Buffered Saline) provides researchers with a reliable and consistent solution essential for various molecular and cellular biology applications. This meticulously prepared buffer is formulated to maintain a stable pH and osmolarity, ensuring optimal conditions for cell culture, protein assays, immunohistochemistry, and many other biological techniques. With its phosphate-based formulation, CellPro™ PBS offers excellent buffering capacity, making it suitable for maintaining the physiological pH of biological samples and preserving their integrity. Whether used for cell washing, sample dilution, or buffer exchange, CellPro™ PBS guarantees exceptional performance and reproducibility in every experiment. Trust in the quality and reliability of CellPro™ PBS to support your research needs and enable precise and consistent results.
Research Products International Corp TRIS BASE, 40% SOLUTION, 4 LITER
A 40% weight by volume solution of TRIS (3.3M TRIS Base) prepared in Molecular Grade Water.
Research Products International Corp MES, Anhydrous, 250 Grams
- CAS Number: 4432-31-9
- Chemical Formula: C6H13NO4S
- Molecular Weight: 195.24 g/mol
Research Products International Corp TRIS-Glycine-SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
Composition:
TRIS (Hydroxymethyl) Aminomethane: < 20.0%
SDS: < 6.0%