Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences CHES BioUltra, >=99.5% (T) | 103-47-9 | MFCD00003835 | 50G
CHES BioUltra, >=99.5% (T) | Purity: >=99.5% (T) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 50G
![Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M92020-5000.0.JPG-250.jpg)
Research Products International Corp MOPS [3-(N-morpholino)-propanesulfonic acid], 5 Kilograms
MOPS, or 3-(N-Morpholino) propanesulfonic acid, is a zwitterionic buffer. MOPS is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.5 to 7.9, making it suitable for applications requiring slightly acidic to neutral pH conditions.
MOPS is commonly used in protein purification, enzyme assays, nucleic acid research, and electrophoresis techniques where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of MOPS is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
pH Range: 6.5-7.9
![Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660070.jpg-250.jpg)
Alkali Scientific MES Monohydrate [2 (N Morpholino) ethanesulfonic acid], 100 G
MES Monohydrate (2-(N-Morpholino)ethanesulfonic acid) is a biochemical reagent used as a pH buffer in biological and chemical research. The 100-gram volume is ideal for preparing buffer solutions that maintain stable pH levels in the range of 5.5 to 7.5. MES is widely used in molecular biology, particularly in enzyme assays, protein studies, and cell culture, where precise pH control is critical for maintaining reaction conditions. MES is particularly effective at buffering biological systems, including during electrophoresis and chromatography, where pH stability ensures accurate separation and quantification of proteins and nucleic acids.
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 500 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660144.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 500 G
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a highly effective pH buffer, widely used in biochemical and biological research. This 500g volume provides a sufficient quantity for larger scale experiments or laboratories that regularly require buffer solutions. MOPS is most commonly utilized in protein and nucleic acid studies, particularly where consistent pH control is necessary over extended periods. It ensures that the pH remains stable within the ideal range of 7.0–7.5, which is critical for maintaining optimal conditions in enzyme assays, electrophoresis, and cell culture experiments. It is also frequently used in molecular biology, biotechnology, and clinical research protocols.
Alkali Scientific Murashige & Skoog Medium with MES Buffer, 120 Grams of Powder, Makes 25 Liters of Solution
Murashige & Skoog Medium with MES Buffer is a specialized medium formulated for plant tissue culture, supplied in a 25-liter format. With 120 grams of powder, this formulation makes 25 liters of nutrient solution. It is enhanced with MES buffer, which ensures that the pH remains stable throughout the culture process, providing optimal conditions for plant cell growth. This medium is ideal for large-scale plant tissue culture studies and experiments. The MES buffer stabilizes the pH, preventing fluctuations that can affect cellular processes, making it essential for consistent growth in laboratory and research environments. This medium is commonly used in biotechnology and plant regeneration projects.
Medchemexpress LLC HEPPS | 16052-06-5 | 99.0% | 25 G
HEPPS (EPPS) is a buffering agent with a useful pH range from 7.3 to 8.7. It reduces Aβ-aggregate-induced memory deficits and rescues cognitive deficits in mice. EPPS is orally active and penetrates the blood-brain barrier.
- Buffering agent with a useful pH range from 7.3 to 8.7.
- Reduces Aβ-aggregate-induced memory deficits.
- Rescues cognitive deficits in mice.
- Orally active.
- Penetrates the blood-brain barrier.
Medchemexpress LLC PIPES (monosodium) | 10010-67-0 | 99.6% | 324.35 | 25 G
PIPES (1,4-Piperazinediethanesulfonic acid) monosodium is a pH buffer that can be mixed with another disodium salt form of PIPES. By changing the ratio and total amount of the PIPES monosodium and disodium buffers, the pH and ionic strength of the medium can be changed.
- pH buffer
- Can be mixed with disodium salt form of PIPES to adjust pH and ionic strength
- For research use only
Medchemexpress LLC Pipes (monosodium) | 10010-67-0 | 99.6% | 324.35 | 10 G
PIPES monosodium (1,4-Piperazinediethanesulfonic acid monosodium) is a pH buffer. It can be mixed with the disodium salt form of PIPES to adjust the pH and ionic strength of the medium by altering the ratio and total amounts of the buffers. This product is intended for research use only.
- Functions as a pH buffer
- Can be mixed with disodium salt form of PIPES
- Allows adjustment of pH and ionic strength
- Intended for research use only
Mitegen HEPES/ NaOH, 1M, pH7.0, 250 ml, CAS # 7365-45-9
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Boston Bioproducts Inc HEPES Buffer (0.5 M, pH 7.5) - 100ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Dojindo Molecular Technologies Inc BES (100G), Good's buffer, CAS: 10191-18-1, pka=7.15, Active pH 6.6-8, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Gold Biotechnology Inc HEPES Free Acid 5 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions