Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659912.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 5 Kg
HEPES, Sodium Salt is a versatile biochemical reagent used to maintain a stable pH in biological and chemical applications. This 5kg package is ideal for large-scale laboratory use, providing ample quantities for high-volume buffer preparation. HEPES sodium salt is effective in the pH range of 6.8–8.2, making it suitable for a wide range of applications including cell culture, protein analysis, and enzymatic reactions. Its high buffering capacity and low toxicity to cells make it a reliable reagent for maintaining physiological pH during extended experiments. This bulk size ensures that laboratories can perform large-scale biochemical and biological studies with consistent results.
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 10 MM x 1 ML
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- Zwitterionic buffer.
- Working pH range of 7.1-8.5.
- Relatively high ability to bind copper(II).
- pKa of 7.84 at 2.0 mM buffer concentration.
Hampton Research HR2-587/0.5 M MES monohydrate - 100 ml
HR2-587/0.5 M MES monohydrate - 100 ml. Please note items from this supplier are non-returnable.
Selleck Chemical LLC Bicine
Bicine (N N-Bis(2-hydroxyethyl)glycine Diethylolglycine Diethanol glycine Dihydroxyethylglycine) is an organic compound used as a buffering agent
![Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660034.jpg-250.jpg)
Alkali Scientific MOPS, Sodium Salt [3 (N Morpholino) propanesulfonic acid sodium salt], 1 Kg
MOPS (3-(N-Morpholino)propanesulfonic acid sodium salt) is a pH buffering reagent commonly used in biological and chemical research. This 1 kilogram bottle provides a larger quantity of MOPS sodium salt for high-volume applications requiring consistent pH stability. It is ideal for maintaining physiological pH in biochemical and biological experiments, such as enzyme assays, protein analyses, and cell culture maintenance. The reagent is particularly useful in experiments that involve prolonged reaction times, as it ensures that pH remains within a narrow, optimal range for sensitive biological systems. MOPS is also used in molecular biology for electrophoresis and other lab protocols that require precise pH control.
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 25G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 25G
![eMolecules 13113-08-1 | N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502174712.JPG-250.jpg)
eMolecules 13113-08-1 | N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193
N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | 13113-08-1 | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193