Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Med Vet International Zoetis Albon, 125mg, 200ct.
VET LICENSE NEEDS TO BE PROVIDED IN 3-4 BUSINESS DAYS OR ORDER WILL BE CANCELLED
125mg Albon (sulfadimethoxine) from Zoetis effectively treats a broad range of bacterial infections commonly found in dogs and cats. This product is indicated for use in dogs and cats with respiratory, genitourinary tract, and soft-tissue infections.
Chem-Impex International, Inc. Piperazine-N,N'-bis(2-ethanesulfonic acid) 1.5 sodium salt | 100037-69-2 | MFCD00066393 | 250G
Piperazine-N,N'-bis(2-ethanesulfonic acid) 1.5 sodium salt, 100037-69-2, MFCD00066393, 250G
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 25G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 25G
Chem-Impex International, Inc. 2-Morpholinoethanesulfonic acid | 4432-31-9 | MFCD00006181 | 100G
2-Morpholinoethanesulfonic acid, 4432-31-9, MFCD00006181, 100G
Chem-Impex International, Inc. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid | 7365-45-9 | MFCD00006158 | 1KG
4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid, 7365-45-9, MFCD00006158, 1KG
Teknova 2.5M Tris-HCl pH 7.5. Endotox
2.5M Tris-HCl, pH 7.5. Endotoxin Tested. 1000mL, Sterile. 6 pack.
Teknova 1M Tris-HCl, pH 8.0, 0.5mL, 50 Tubes per Pack, Sterile. 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Apexbio Technology LLC Annexin V-PE/7-AAD Apoptosis Kit 20tests
Annexin V-PE/7-AAD Apoptosis Kit enables differentiation between early apoptotic late apoptotic and dead cells Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane phospholipid bilayer However during the early stages of apoptosis PS moves from the inner surface to the outer surface of the cell membrane becoming exposed to the extracellular environment Labeled with a fluorescence dye such as PE Annexcin V is commonly used as a fluorescent probe to detect apoptosis through flow cytometry or fluorescence microscopy within 15-30 minutes In addition this kit includes 7-AAD a nucleic acid dye that specifically binds double-stranded DNA and emits red fluorescence Normally 7-AAD cannot permeate intact cell membranes however it can penetrate compromised membranes of late apoptotic and dead cells staining their nuclei
Medchemexpress LLC Pipes (monosodium) | 10010-67-0 | 99.6% | 324.35 | 10 G
PIPES monosodium (1,4-Piperazinediethanesulfonic acid monosodium) is a pH buffer. It can be mixed with the disodium salt form of PIPES to adjust the pH and ionic strength of the medium by altering the ratio and total amounts of the buffers. This product is intended for research use only.
- Functions as a pH buffer
- Can be mixed with disodium salt form of PIPES
- Allows adjustment of pH and ionic strength
- Intended for research use only
Alkali Scientific CellPro™ Tris Glycine SDS Buffer 10X, pH8.3, 1L x 12
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.