Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-EDTA |
| Grade | Molecular Biology |
| DNase | DNase free |
| Filtered Through | Filtered through a 5-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 1X Solution, pH 8.0 |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| Health Hazard 2 | CAUTION! |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| Recommended Storage | RT |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
| CAS | 10043-35-3 |
|---|---|
| Synonym | TBE |
TAE Buffer, Tris-Acetate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents
| Name Note | 10X Solution |
|---|---|
| Concentration or Composition (by Analyte or Components) | Tris (<5.0%), Acetic Acid (1.0%), and EDTA (<1%). |
| CAS | 77-86-1 |
| Protease | Protease free |
| Physical Form | Liquid |
| Chemical Name or Material | Tris-Acetate-EDTA |
| Grade | Electrophoresis |
| Synonym | TAE |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| DNase | DNase free |
| Recommended Storage | RT |
| Filtered Through | Filtered through a 0.2-micron filter. |
| ChemAlert Storage Symbol | Gray |
TE Buffer, 1X Solution pH 8.0, Low EDTA, Molecular Biology Grade
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
Tris-HCl, 1M Solution, pH 8.0, Molecular Biology Grade, Ultrapure
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
| Packaging | Plastic bottle |
|---|---|
| Physical Form | Liquid |
| Recommended Storage | Ambient temperatures |
| Color | Undesignated |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-Glycine-SDS |
| Grade | Electrophoresis |
| Identification | Pass Test |
| DNase | DNase free |
| Filtered Through | Filtered through a 0.2-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 10X Solution |
| Concentration or Composition (by Analyte or Components) | 0.25M Tris Base, 1.92M Glycine, and 1.0% (w/v) SDS. |
| CAS | 151-21-3 |
| Protease | Protease free |
| Synonym | TG-SDS |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
| Packaging | Plastic bottle |
|---|---|
| Physical Form | Liquid |
| Recommended Storage | Ambient temperatures |
| Color | Undesignated |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-Glycine |
| Grade | Electrophoresis |
| DNase | DNase free |
| Filtered Through | Filtered through a 0.2-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 10X Solution |
| Concentration or Composition (by Analyte or Components) | Components: Tris (2-3%) and Glycine (13-14%). 0.25M Tris Base and 1.92M Glycine. |
| CAS | 56-40-6 |
| Protease | Protease free |
| Synonym | TG |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
Thermo Scientific™ Sodium cacodylate, 0.1M buffer soln., pH 7.0
CAS: 6131-99-3 Molecular Formula: C2H6AsNaO2 Molecular Weight (g/mol): 159.98 MDL Number: MFCD00149079 InChI Key: IHQKEDIOMGYHEB-UHFFFAOYSA-M IUPAC Name: sodium dimethylarsinate SMILES: [Na+].C[As](C)([O-])=O
| CAS | 6131-99-3 |
|---|---|
| Molecular Weight (g/mol) | 159.98 |
| MDL Number | MFCD00149079 |
| SMILES | [Na+].C[As](C)([O-])=O |
| IUPAC Name | sodium dimethylarsinate |
| InChI Key | IHQKEDIOMGYHEB-UHFFFAOYSA-M |
| Molecular Formula | C2H6AsNaO2 |
Thermo Scientific Chemicals MES, 1.0M buffer soln., pH 6.0
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.25 |
| MDL Number | MFCD00149409 |
| SMILES | O.OS(=O)(=O)CCN1CCOCC1 |
| IUPAC Name | 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Thermo Scientific Chemicals MES, 1.0M buffer soln., pH 5.0
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.25 |
| MDL Number | MFCD00149409 |
| SMILES | O.OS(=O)(=O)CCN1CCOCC1 |
| IUPAC Name | 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Gibco™ CTS™ DPBS, calcium, magnesium
Gibco Cell Therapy Systems DPBS CTS™ is the classical Dulbecco's Phosphate Buffered Saline which contains calcium, magnesium but no phenol red.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.4
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |