Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc TE pH 7.4, 1000 mL
TE is composed of buffering agent Tris, and chelating agent EDTA. ¿ EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). ¿ Together, this Tris-EDTA based solution solubilizes DNA or RNA, while protecting it from degradation. ¿ Applications: TE is useful for reconstituting nucleic acids, during DNA precipitation and other DNA applications, storing and diluting DNA and RNA, breaking protein-links in immuno-histochemistry procedures, and unmasking antigens and epitopes in formalin-fixed and paraffin-embedded tissue sections. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Quality Biological Inc Tris HCl (1 M), pH 7.5, 100 mL
Tris is a buffer component in molecular biology, tissue culture, and electrophoresis procedures. ¿ These solutions of Tris Hydrochloride provide convenient and highly purified solutions. ¿ Applications: Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). ¿ Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells. ¿ Storage Conditions: 15¿C to 30¿C ¿ Shipping Condition: ambient temperature ¿ Sterile Filtered ¿ DNase, RNase and Protease Tested ¿ pH Range: 7.5 ¿ 0.1 ¿ Product and/or packaging contains recycled/renewable content ¿ Product and/or packaging is recyclable or reduces waste ¿ Product and/or packaging is safer to human and environmental health ¿ ¿ Max Shelf Life: 36 months
Gold Biotechnology Inc HEPES Free Acid 2 x 1 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Kd Medical Inc PBS Solution With Sodium Azide, 500 mL, Antimicrobial Preservative
PBS W/SODIUM AZIDE 500ML is a buffered saline solution designed for laboratory applications requiring a preservative to inhibit microbial growth. This product not only provides a stable wash and storage buffer but also enhances the longevity of biological samples. The inclusion of sodium azide ensures effective preservation, differentiating it from standard saline solutions. This versatile product is essential for researchers seeking reliable and long-lasting buffer solutions.
![eMolecules 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925](https://assets.fishersci.com/TFS-Assets/CCG/product-images/NC3454793.JPG-250.jpg)
eMolecules 2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
2-(N-MORPHOLINO)ETHANESULFONIC ACID POTASSIUM SALT | AstaTech | 39946-25-3 | MFCD00069752 | 233.320 | C6H12KNO4S | 95.000 | [K+].[O-]S(=O)(=O)CCN1CCOCC1 | 100g | 696752925
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerforman1L
MOPS SOLUTIONon BioPerforman1L
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Sigma Aldrich Fine Chemicals Biosciences MES sodium salt 99 titrati25G
MES sodium salt is commonly employed as buffer. Useful pH range for MES sodium salt is 5.5 to 6.7.
Sigma Aldrich Fine Chemicals Biosciences TRIS Glycine buffer soluti1L
TRIS Glycine buffer SOLUTION1L
Sigma Aldrich Fine Chemicals Biosciences TrizmaR base Primary Stand25KG
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations such as Tris-EDTA (TE) buffer Tris-acetate-EDTA (TAE) buffer Tris-borate-EDTA (TBE) buffer etc. It is pure essentially stable relatively non-hygroscopic and has a high equivalent weight.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
This DMEM-Hi glucose medium is a 1x complete medium with sodium pyruvate added. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Phosphate Bufer500ML
Dulbeccos Phosphate Bufer500ML