Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.5 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline
PHOSPHATE Phosphate buffered saline
Chemscene ChemScene | Boc-Piperazine-OH | 25G | CS-W009135 | 0.98 | 77279-24-4| MFCD00728947 | 230.3
ChemScene | Boc-Piperazine-OH | 25G | CS-W009135 | 0.98 | 77279-24-4| MFCD00728947 | 230.3
Teknova 1M Tris-HCl Solution, pH 7.4. 500 ml, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1M Tris-HCl, pH 9.5. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Rockland Immunochemicals 10X PBS pH 7.2 with Azide (0.2 M Potassium Phosphate 1.5 M Sodium Chloride 0.1% (w/v) Sodium Azide) - MB-011
Phosphate Buffered Saline (PBS) is a regularly used buffer in biological research. The phosphate ions help maintain a constant pH around neutral. The osmolarity of solution typically matches that of the human body (isotonic solution). PBS can act as a diluent for many experimental preparations and is non-toxic to cells. This preparation contains 0.1% sodium azide as a preservative and is 10X concentrated to be diluted 10-fold to the working concentration.
Teknova 1.5M Tris-HCl, pH 8.8. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences MOPS solution BioPerforman1L
MOPS SOLUTIONon BioPerforman1L
LAB BIOREAGENTS NGB III Buffer 250ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.
Bioworld HEPES, 1M, pH 7.4, Sterile, 250 mL
HEPES BufferAn N-substituted aminosulfonic acid buffer with an optimal buffering pH range of 6.0 to 8.5 Technical Information:Biotechnology GradeDNase(P/F): NoneProtease(P/F): NoneRNase(P/F): NoneAppearance: LiquidBoiling Point : 210-215 °CDensity : 1.33 g/cm3 (Predicted)Melting Point: 234 °C (dec.)PH Value: 7.4RTECS Number: TL6809000HS Code:2933.59.95
Boston Bioproducts Inc Tris-Borate-EDTA Buffer (10X) - 4L
The Tris-Borate-EDTA (TBE) Buffer is a pH regulator and chelator used in molecular biology techniques such as nucleic acid gel electrophoresis. Supporting a pH range between 8.1 and 8.5, the TBE Buffer combines aqueous tris base, boric acid, and EDTA (Ethylenediaminetetraacetic Acid), making it efficient as a running buffer or component of agarose gels. This product is prepared in a 10X stock concentration, and can be diluted to concentrations ideal to specific laboratory workflows.
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.4) - 1L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics physiological osmolarity and ion concentrations, making it ideal for applications including protein purification, Western blotting and immunoassays, sample dilution, and as a transport medium. Packaged as a 10X concentration, this solution can be diluted to support its application.