Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
ILEX LIFE SCIENCES LLC 2-MERCAPTOETHANOL 50MM 100ML
PAN-Biotech 2-Mercaptoethanol 50 mM in DPBS (100 ml). DESCRIPTION: 2-Mercaptoethanol, also known as beta-mercaptoethanol (BME), is a potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals. 2-Mercaptoethanol is not stable in solution, thus protocols generally require daily supplementation. PAN-Biotech 2-Mercaptoethanol is supplied at a concentration of 50 mM in Dulbecco's Phosphate Buffered Saline (DPBS). SOLUTION: 50 mM in DPBS. STORAGE: +2-8°C. STERILE: Yes. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany.
Bioworld PBS-Casein, 0.5%, 200 mL
Supplied in PBS as 0.5% casein ready for use.pH range 7.4 (+/- 0.1)Sold as 2 x 100mL or 500mL.
Bioworld PIPES BUFFER 1M PH 6.0 100ML
liquid, 1.0m, ph 6Synonym(s): 1,4-Piperazinediethanesulfonic acidMolecular Formula: C8H18N2O6S2CAS Number: 5625-37-6Molecular Weight: 302.37
Medchemexpress LLC Heppso | 68399-78-0 | 99.89% | 10 MM x 1 ML
HEPPSO is a zwitterionic buffer with a working pH range of 7.1-8.5. It exhibits a relatively high ability to bind copper(II) and has a pKa of 7.84 at a 2.0 mM buffer concentration.
- Zwitterionic buffer.
- Working pH range of 7.1-8.5.
- Relatively high ability to bind copper(II).
- pKa of 7.84 at 2.0 mM buffer concentration.
Chem-Impex International, Inc. 3-Cyclohexylamino-1-propanesulfonic acid | 1135-40-6 | MFCD00003837 | 25G
3-Cyclohexylamino-1-propanesulfonic acid, 1135-40-6, MFCD00003837, 25G
![eMolecules 13113-08-1 | N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193](https://assets.fishersci.com/TFS-Assets/CCG/product-images/502174712.JPG-250.jpg)
eMolecules 13113-08-1 | N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193
N-(3-Indolylacetyl)glycine | Toronto Research Chemicals | 13113-08-1 | MFCD00079392 | 232.239 | C12H12N2O3OC(=O)CNC(=O)Cc1c[nH]c2ccccc12 | 250mg | 601597193
Medchemexpress LLC Ada disodium | 41689-31-0 | 98.0% | 10 G
ADA (disodium) is a buffer salt derived from ADA (adenosine deaminase) and is widely used in biochemical research, especially in the preparation of enzyme buffers. It is for research use only.
- Buffer salt derived from adenosine deaminase
- Widely used in biochemical research
- Used in the preparation of enzyme buffers
![Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 25 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660132.jpg-250.jpg)
Alkali Scientific POPSO, Free Acid, [Piperazine N N' bis(2 hydroxypropane sulfonic acid)], 25 G
POPSO (Free Acid), Laboratory Grade, is available in a 25g package and is used in a wide range of biochemical and molecular biology applications. It is commonly used as a buffering agent to maintain a stable pH environment during protein analysis, enzyme assays, and other scientific procedures. POPSO is known for its effectiveness in maintaining physiological pH ranges, making it ideal for cell culture, biochemical reactions, and analytical studies. The reagent is available in a convenient 25g size, perfect for smaller experiments and academic research, ensuring reliable performance in maintaining pH stability during laboratory work.
![Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660183.jpg-250.jpg)
Alkali Scientific HEPES, Sodium Salt [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 1 Kg
HEPES, Sodium Salt is a widely used biochemical reagent for maintaining pH stability in biological and chemical applications. This 1kg package is perfect for laboratories that require larger quantities of buffer for cell culture, protein analysis, or drug formulation. HEPES sodium salt is effective in the pH range of 6.8–8.2, ensuring that experiments are conducted under optimal conditions. The reagent's excellent buffering capacity and low toxicity to cells make it ideal for long-term cell culture and biochemical assays, providing reliable results and maintaining physiological pH for sensitive biological processes.
Dojindo Molecular Technologies Inc PIPES (25G), Good's buffer, CAS: 5625-37-6, pka=6.8, Active pH 6.1-7.5, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.