Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC Iminodiacetic acid | 142-73-4 | 99.9% | 133.10 | 100 G
Iminodiacetic acid | 142-73-4 | 99.9% | 133.10 | 100 G
![Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659565.jpg-250.jpg)
Alkali Scientific PIPES [Piperazine N N' bis(2 ethanesulfonic acid)], USP, 100 G
PIPES [Piperazine N N' bis(2 ethanesulfonic acid)] is a biochemical reagent supplied in USP grade, specifically formulated for use in biological and chemical research. This 100g bottle is ideal for preparing solutions where pH stability is required. PIPES is commonly used as a buffer in a wide range of biochemical experiments due to its ability to maintain a stable pH, especially under conditions where other buffers may fail. It is used in protein studies, enzyme kinetics, and various biochemical assays. The buffer provides effective pH control in the physiological range, making it a versatile tool in molecular biology, immunology, and other laboratory disciplines.
![Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659757.jpg-250.jpg)
Alkali Scientific HEPES, Free Acid [N (2 Hydroxyethyl) piperazine N' (2 ethanesulfonic acid)], 1 Kg
HEPES, Free Acid (N-(2-Hydroxyethyl) Piperazine-N'-(2-Ethanesulfonic Acid)) is a high-quality biochemical reagent widely used as a buffer in biological and chemical research. The 1kg size is ideal for large-scale research laboratories that require a stable, high-purity buffer for routine use. HEPES is particularly useful in maintaining consistent pH levels in cell culture, enzyme assays, and protein analysis, where precise control of pH is critical. As a zwitterionic buffer, it provides excellent pH stability in the physiological range without interfering with biological reactions, making it an essential tool in a variety of biochemical and molecular applications.
Alkali Scientific MES, Anhydrous, 25 G
MES, Anhydrous, 25 G, is a small-volume, high-purity buffering agent used to maintain pH stability in laboratory experiments. With its anhydrous form, it dissolves readily in solvents for precise pH adjustments. MES buffers in the pH range of 5.5 to 6.7, making it particularly useful in applications such as electrophoresis, enzyme assays, and in preparing cell culture media. The 25 g package is ideal for small-scale experiments or for laboratories that require frequent pH adjustments but do not need larger quantities of the reagent. MES is an essential tool for many biochemical and biological processes that require exacting conditions for reproducible results.
Medchemexpress LLC 4-Morpholinepropanesulfonic acid, sodium salt (1:1) | 71119-22-7 | 99.8% | 50 G
MOPS sodium salt is a buffering agent utilized in biological applications. It effectively maintains the pH of mammalian cell culture media. Additionally, MOPS sodium salt has been shown to significantly enhance the thermal stability of BSA (Bovine Serum Albumin).
- Buffering agent for biological applications.
- Maintains the pH of mammalian cell culture media.
- Significantly enhances the thermal stability of BSA.
AG Scientific Inc MOPS Free Acid 1 KG
MOPS, also known as 3-(N-morpholino)propanesulfonic acid, is one of Good’s buffers. It is N-substituted amino sulfonic acid with a morpholinic ring and has zwitterionic and inert properties. MOPS buffer is broadly used in biological and biochemical studies. It can be mixed with MOPS sodium salt to attain desired pH.MOPS is recommended as an agent for non-coordinating buffers in solutions with metal ions because it hardly forms a complex with most metal ions such as Cu, Ni, Mn, Zn and Co ions. The Good's buffer interferes with the Folin protein assay and partially decomposes when autoclaved in the presence of glucose. MOPS can be used as a Good’s buffer because it has low UV absorptivity, minimal reactivity, stable pH and is soluble in water.