Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific Tris Acetate Ultra Pure, 500 G
Tris Acetate Ultra Pure is a high-quality buffering agent formulated for high-precision laboratory applications. With its ultra-pure grade, it ensures minimal interference in sensitive experiments, maintaining a stable pH environment. This 500g bottle is ideal for medium-scale preparations, supporting a wide range of biochemical and molecular biology applications, including electrophoresis, protein assays, and enzyme reactions. The tris acetate buffer is particularly effective for experiments requiring consistent pH conditions, making it a reliable choice for researchers working in protein analysis, DNA/RNA extraction, and various other scientific fields where precision is critical.
Crescent Chemical Co Inc SERVA TRIS-TRICINE/SDS BUFFER
SERVA Tris-Tricine/SDS electrophoresis buffer (10x) Running buffer for SDS PAGE. Supplied as 10 x concentrate. Contains 1 M Tris, 1 M Tricine and 1 % SDS. Size - 1L Storage Conditions - +15 °C TO +30 °C Catalog Number - 42552.01
Bioworld TAE Buffer, Quick-Mix Pack (25X), 1 PK(s)
Product DescriptionTAE (Tris-Acetate-EDTA) buffer quick-mix pack prepares 1 L of 25X concentration. This solution has a pH level between 8.2 – 8.4 and is stored at room temperature.TAE is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. It can be used for both genomic and large supercoiled DNA, and can also be used as both a running buffer and a gel preparation buffer. It is recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA. TAE buffer has a relatively low buffering capacity and therefore replacement of the buffer during prolonged electrophoresis is recommended.
Bioworld PEB- Protein Extraction Buffer (5x), 250 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
LAB BIOREAGENTS NGB I Buffer 100ml
Lab Bioreagents NGB (I, II, III) are newly developed next generation assay buffers that provides exceptional performance and broad compatibility for Western blot, ELISA, IHC and nucleic acid detection assays. The NGBs are basic buffers that hat requires neutralization by acid in the reaction. Among the tests, NGBs have been demonstrated to maintain the full bioactivity of proteins and antibodies.
Boston Bioproducts Inc Molecular Biology Grade Water - 500ml
Molecular Biology Grade Water - 500ml (For Research Use and Further Manufacturing Only)