Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific QuickSilver™ Buffer Powder, Tris-MOPS-SDS Buffer Powder, 50 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
Alkali Scientific CellPro™ 1X DPBS, Dulbecco’s Phosphate Buffered Saline, without Calcium or Magnesium, Sterile Filtered, 500mL, 10/Case
Like PBS, DPBS is also a water-based solution containing sodium chloride and phosphate buffer that is used in biological applications. However, DPBS differs from PBS in that it also includes potassium chloride and is available in a larger variety of formulations.
Hampton Research HR2-781/1.0 M BIS-TRIS pH 5.5 - 100 ml
HR2-781/1.0 M BIS-TRIS pH 5.5 - 100 ml. Please note items from this supplier are non-returnable.
Alkali Scientific CellPro™ Tris Buffer-HCL, 1M, pH 8.0, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Gold Biotechnology Inc MES Free Acid 250 g
MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 5 5 6 7 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MES lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions MES is often used in buffered culture media for bacteria yeast and mammalian cells It is toxic towards plants at high concentrations but can be used in culture media at lower concentrations for plants Due to low ionic mobility MES is regarded as an excellent buffer for use in capillary electrochromatography MES is often employed as a substitute for the highly toxic cacodylate as well as a substitute for the non-zwitterionic buffers citrate and malate
Gold Biotechnology Inc MOPS Free Acid Ultra Pure
MOPS is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic morpholinic buffer that is useful for a pH range of 6 5 7 9 and commonly used for cell culture media as a running buffer in electrophoresis and for protein purification in chromatography MOPS lacks the ability to form a complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Alkali Scientific CellPro™ Tris Buffer, 2M, pH 7.5, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ Sterile Saline 0.9%, 100mL
Introducing CellPro™ Sterile Saline, meticulously crafted by Alkali Scientific™ to meet the demanding standards of molecular biology applications. This Molecular Biology Grade saline solution ensures exceptional purity and sterility, making it ideal for various laboratory procedures, including cell culture, molecular assays, and biochemical experiments. With its precisely balanced formulation, CellPro™ Sterile Saline provides the essential ions and osmotic balance required for cell viability and physiological studies. Each batch undergoes rigorous quality control to guarantee consistency and reliability, empowering researchers with confidence in their results. Trust CellPro™ Sterile Saline to support your molecular biology research with unparalleled quality and performance.
Alkali Scientific CellPro™ Sterile Saline, 0.9%, 1L, 10/cs
Introducing CellPro™ Sterile Saline, meticulously crafted by Alkali Scientific™ to meet the demanding standards of molecular biology applications. This Molecular Biology Grade saline solution ensures exceptional purity and sterility, making it ideal for various laboratory procedures, including cell culture, molecular assays, and biochemical experiments. With its precisely balanced formulation, CellPro™ Sterile Saline provides the essential ions and osmotic balance required for cell viability and physiological studies. Each batch undergoes rigorous quality control to guarantee consistency and reliability, empowering researchers with confidence in their results. Trust CellPro™ Sterile Saline to support your molecular biology research with unparalleled quality and performance.
Alkali Scientific CellPro™ TE 1X (Tris EDTA), pH7.4, 100mL
Introducing CellPro™ TE 1X (Tris EDTA), meticulously formulated by Alkali Scientific™ to meet the exacting standards of molecular biology research. This Molecular Biology Grade TE buffer ensures exceptional purity and performance, making it an indispensable tool for various molecular biology applications, including DNA and RNA purification, DNA sequencing, and PCR. With its precisely balanced composition and stringent quality control measures, CellPro™ TE 1X provides researchers with reliable and reproducible results, facilitating accurate and efficient laboratory workflows. Trust CellPro™ TE 1X for your molecular biology experiments and experience the assurance of superior quality and performance in every assay.
Alkali Scientific CellPro™ TAE, 10X (Tris acetate EDTA), 4L Cubitainer
Alkali Scientific™ CellPro™ TAE Tris Acetate EDTA is a fundamental reagent in nucleic acid electrophoresis, renowned for its exceptional buffering capacity and DNA solubilization properties. Designed to provide optimal performance and reproducibility, this solution enables precise separation and analysis of DNA fragments in agarose gel electrophoresis. Each batch undergoes rigorous sterile filtration to ensure purity and consistency, empowering researchers with confidence in their molecular biology experiments.
Alkali Scientific CellPro™ Tris-HCL, 1M, Stock Solution, pH 8.8, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Alkali Scientific CellPro™ Tris-Glycine SDS Running Buffer, 10X, pH 8.3, 100mL
Alkali Scientific™ CellPro™ Tris Glycine offers a sterile solution ideal for a variety of biochemical and molecular biology applications. Composed of Tris (Tris(hydroxymethyl)aminomethane) and Glycine, this buffer provides optimal conditions for protein electrophoresis, particularly in SDS-PAGE (Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis) systems. The sterile formulation ensures the purity and integrity of your samples, minimizing the risk of contamination and ensuring reliable results. CellPro™ Tris Glycine buffer is versatile and suitable for use in protein separation, transfer, and analysis techniques, making it an essential component in the laboratory for protein research and characterization. Trust in the quality and consistency of CellPro™ Tris Glycine for your protein electrophoresis experiments.
Alkali Scientific CellPro™ Tris HCl, 1M, pH 8.0, 500mL
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Bioworld EGTA 0.5M, PH 8.0,250ML
EGTA 0.5M, pH 8.0, DNase and RNase Free,250ml.EGTA Buffer is of a 0.5M concentration and pH of 8.0 that is DNase and RNase free. EGTA Buffer is a metal chelating agent specific for calcium ions. EGTA Buffer is commonly used in tissue culture media and biological buffers.