Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld Tris-Glycine (TG) Running Buffer (10X), 4 L
Product DescriptionTris-Glycine (TG) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris base and 0.192M Glycine. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated using bioPlus Tris powder to ensure exceptional purity along with the absence of nuclease and protease activities. TG Buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Research Products International Corp TRIS BUFFERED SALINE, 10X SOLUTION, 4 LITER
TBS 10X Solution (Tris Buffered Saline) is a concentrated buffered salt solution containing Tris and sodium chloride. It maintains pH stability and provides ionic strength for biological experiments. Tris acts as a buffering agent to keep pH consistent, while sodium chloride mimics physiological conditions. Dilute 10X solution with distilled water to prepare working concentrations (e.g., 1X). TBS is widely used in protein and nucleic acid blotting, immunohistochemistry, and molecular biology techniques, ensuring stable and reproducible results across diverse applications.
![Research Products International Corp TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T60015-1000.03.JPG-250.jpg)
Research Products International Corp TAE 50X Buffer [TRIS Acetate EDTA 50X Solution], 1 Liter
TAE 50X Buffer is a concentrated solution of Tris, acetic acid, and EDTA used in nucleic acid electrophoresis. When diluted, it maintains pH (~8.3) and low ionic strength, supporting clear DNA/RNA separation. EDTA chelates metal ions, helping prevent nucleic acid degradation during electrophoretic analysis in molecular biology workflows.
Substance Name: TRIS Acetate EDTA 50X Solution
Solution contains:
Acetic Acid: 1 M
EDTA, Disodium Salt Dihydrate: 50 mM
Alkali Scientific QuickSilver™ Buffer Powder, TAE Buffer Powder, 100 pouches
QuickSilverª Buffer Powder Packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, easy to use, and come in a variety of buffers for Nucleic Acid and Protein Electrophoresis, Western Blotting, ELISA, and Cell Culture. Quick and Economical (and low shipping costs!) Convenient premeasured pouches Each pouch makes 1L of 1X buffer No pH adjustment needed Nuclease and protease free Quality and consistency guaranteed Long shelf life Buffers for Electrophoresis of Nucleic Acids and Proteins: TBE, TAE, Fast Running Buffer, Tris-Glycine-SDS, Tris-MOPS-SDS, Tris-MES-SDS Buffers for Western Blotting, ELISA and Cell Culture: TBS, TBST, PBS, PBST
![eMolecules 1214727-57-7 | tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000158799.jpg-250.jpg)
eMolecules 1214727-57-7 | tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276
tert-Butyl (trans-3-(aminomethyl)cyclobutyl)carbamate | Ambeed | 1214727-57-7 | MFCD22123684 | 200.282 | C10H20N2O2 | 98.000 | CC(C)(C)OC(=O)N[C@H]1C[C@H](CN)C1 | 1g | 575777276
Boston Bioproducts Inc Ethanol (20%) - 2x1L
Bioprocess Grade - Ethanol (20%) - 2x1L (For Research Use and Further Manufacturing Only)
Alkali Scientific SSC Buffer 20X Powder, 263.6 Grams of Powder, Makes 1 Liter of 20X Solution
SSC Buffer 20X Powder (263.6 Grams) is a concentrated solution commonly used in DNA hybridization experiments. The powder form allows labs to prepare the solution in the exact volume needed by dissolving it in distilled water. This 20X concentrated solution is widely used in molecular biology for nucleic acid hybridization, particularly in Southern and Northern blotting, where high salt concentrations help in stabilizing the probe-target interactions. The SSC buffer helps maintain an optimal environment for hybridization, ensuring that DNA or RNA strands bind specifically to their complementary sequences during experimentation. This 1-liter solution is critical for efficient and accurate nucleic acid analysis.
Alkali Scientific SSC Buffer, 20X Solution, 1 L
SSC Buffer 20X Solution (1 L) is a ready-to-use, concentrated buffer solution widely used in DNA hybridization experiments. This 20X concentration allows for easy dilution to the desired working concentration in hybridization assays, including Southern and Northern blotting. The high ionic strength provided by SSC Buffer stabilizes nucleic acid interactions, ensuring that probes bind specifically to complementary sequences during hybridization. The 1-liter volume is suitable for routine laboratory use, offering a convenient quantity for hybridization experiments and nucleic acid detection protocols. It is an essential reagent in molecular biology for accurate and reliable results.
Boston Bioproducts Inc HBS-EP+ Buffer (10X, pH 7.4) - 4x500ml
HBS-EP Plus Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)