Copper Fluor-4 (CF4) is a Cu+-specific fluorescent probe based on a rhodol dye scaffold. It exhibits high selectivity for copper (Kd value of 2.9x10-13 M), especially over zinc, iron, and various cellular alkali and alkaline earth metals. The probe maintains stability within a physiologically relevant pH range of 6 to 8, with excitation at 415 nm and emission at 660 nm.
- Specific fluorescent probe for Cu+ ions
- Developed from a rhodol dye scaffold
- Offers high selectivity for copper, including over zinc and iron
- Maintains stability across a pH range of 6 to 8
- Excitation at 415 nm
- Emission at 660 nm
- Can localize copper in the soma region of PC-12-derived neurons
- Suitable for assessing labile copper distributions in living organisms like zebrafish embryos
- Monitors labile Cu pools through reversible Cu binding in worms