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Sparfosic acid is N-phosphonacetyl-L-aspartic acid, a DNA antimetabolite and potent inhibitor of aspartate transcarbamoyl transferase (ATCase). It is used in biochemical and cell-based research to disrupt de novo pyrimidine biosynthesis and has been reported to increase the cytotoxic effect of 5-fluorouracil combined with interferon-alpha in cancer cell models.