Carbonyl compounds

Sigma Aldrich Fine Chemicals Biosciences 5 Bromo 2 deoxy uridine La

The kit is used for the detection of BrdU incorporated into cellular DNA using immunofluorescence microscopy. It is used for the detection of DNA synthesis by either in vitro labeling of cells or organ cultures or by in vivo labeling in which frozen or paraffin-embedded tissue sections must be prepared prior to fixation.Normally binding of the antibody is only achieved by denaturation of the DNA. This is usually obtained by exposing the cells to acid base or heat. These procedures result in destruction of cell integrity including cell morphology and surface and cytoplasmatic markers.The BrdU Labeling and Detection Kit I avoids these problems. The antibody preparation contains specific nucleases which allows access to BrdU after fixation in acidic ethanol. Therefore also simultaneous detection of other markers (double staining) is possible.

Malonic acid 141-82-2 100mg

Malonic acid is a small-molecule dicarboxylic acid It is commonly used to facilitate the cross-linking of polysaccharide molecules such as corn starch and potato starch by forming covalent linkages via its two carboxyl groups Malonic acid acts primarily through condensation or esterification reactions to increase intermolecular connectivity and modulate the mechanical properties of biopolymer matrices Based on these properties malonic acid holds research potential in pharmaceutical excipient formulation drug delivery system development and biomaterial engineering studies

4-Thio-dTTP 50ul (100 mM)

4-Thio-dTTP is a nucleotide analog structurally similar to thymidine triphosphate (dTTP) It incorporates into DNA strands during enzymatic synthesis modifying nucleic acid interactions and affecting the activities of enzymes involved in DNA replication and repair 4-Thio-dTTP exerts its biological activity through incorporation into DNA enabling the study of DNA polymerase fidelity replication kinetics enzyme-substrate interactions and mechanisms of DNA damage responses Based on these biochemical properties 4-Thio-dTTP holds research potential in applications such as investigating DNA synthesis regulation enzymatic mechanisms and pathways related to cancer and genomic instability

4-Thio-dTTP 100ul (100 mM)

4-Thio-dTTP is a nucleotide analog structurally similar to thymidine triphosphate (dTTP) It incorporates into DNA strands during enzymatic synthesis modifying nucleic acid interactions and affecting the activities of enzymes involved in DNA replication and repair 4-Thio-dTTP exerts its biological activity through incorporation into DNA enabling the study of DNA polymerase fidelity replication kinetics enzyme-substrate interactions and mechanisms of DNA damage responses Based on these biochemical properties 4-Thio-dTTP holds research potential in applications such as investigating DNA synthesis regulation enzymatic mechanisms and pathways related to cancer and genomic instability