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Recombinant human matrix metalloproteinase-9 (MMP-9) expressed in Pichia pastoris and purified with a His tag. Supplied as a lyophilized powder for research use, MMP-9 is involved in localized extracellular matrix breakdown and leukocyte migration. Enzymatic activity testing is in progress and activity is not guaranteed by the manufacturer.
Expression system: P. pastoris.
His-tagged for affinity purification.
Molecular weight: 69 kDa.
Purity greater than 90% by reducing SDS-PAGE.
Endotoxin level below 1 EU/μg (LAL).
Formulation: lyophilized from 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Storage: store at -20°C; stable up to 2 years frozen.
Reconstitution: avoid concentrations below 100 μg/mL in ddH2O; aliquot for long-term storage.
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