Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences CfTM 633 succinimidyl este
far-red fluorescent dyes offer the advantage of ultra sensitive detection because background signal due to autofluorescence in most biological samples is minimal in this spectral region. for many years the cyanine dye Cy(R)5 has been the dye of choice for such detection. More recently Alexa fluor(R) 647 has been developed as a better alternative by having brighter fluorescence and higher photostability. Despite the improvement Alexa fluor(R) 647 still lacks sufficient photostability required for many demanding applications. On the other hand while Alexa fluor(R) 633 is photostable its fluorescence on proteins is very weak. In fact it has been a challenging task for dye chemists to develop a far-red fluorescent dye that is both highly fluorescent and photostable for protein and nucleic acid labeling. Using new chemistry scientists at Biotium have successfully developed a series of far-red and near-IR dyes to overcome these challenges. With its absorption peak at 630 nm Cf(TM)633
Sigma Aldrich Fine Chemicals Biosciences HiTrapR Heparin High Perfo
HiTrap(R) Heparin HP columns are prepacked with Heparin Sepharose(R) High Performance for high-reSOLUTIONon purification of proteinswith affnity forheparin such as DNA-binding proteins coagulation factors lipoproteins and protein synthesis factors.Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules including coagulation factors and other plasma proteins lipoproteins protein synthesis factors enzymes that act on nucleic acids and steroid receptors. Heparin also functions as a high capacity cation exchanger due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.Heparin is coupled to the Sepharose(R) High Performance base matrix via the N-hydroxysuccinamide coupling method to give high capacity performance and low leakage levels. HiTrap(R) Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap(R) H