Recirculating Chillers
Sigma Aldrich Fine Chemicals Biosciences Tris2-carboxyethylphosphin
Tris(2-carboxyethyl)phosphine immobilized onAgarose CL-4B Trialkylphosphines (TCEP) are highly effective agents for reducing disulfide bonds in proteins peptides and other disulfide bondcontaining molecules and are relatively non-reactive toward other functional groups. The trialkylphosphine TCEP was first described by Levison et al. as an odorless and efficient reductant of alkyl disulfides over a wide pH range. TCEP is stable in aqueous SOLUTIONons and does not undergo the rapid oxidation that often occurs with other reducing agents such as dithiotreitol (DTT) and beta-mercaptoethanol (BME 2-ME). TCEP does not interfere with commonly used sulfhydryl-reactive reagents (e.g. maleimide crosslinkers). Nevertheless many protocols require recovery of the reduced sample separate from the reducing agent. Our Tris(2-carboxyethyl)phosphine immobilized on Agarose CL-4B eliminates the need to use laborious and troublesome gel filtration methods to separate the reduced sample from the reduci
Sigma Aldrich Fine Chemicals Biosciences White anti-static disposab
WHITE White anti-static disposab
Sigma Aldrich Fine Chemicals Biosciences L-alpha-Phosphatidyl-DL-gl25MG
L L-alpha-Phosphatidyl-DL-gl25MG
Med Vet International Miltex Superoxol Bleaching Agent, Hydrogen Peroxide, 35%, 1 oz, Each, 018-39800
Superoxol is a dental bleaching agent used to bleach nonvital teeth. 1 oz.
Sigma Aldrich Fine Chemicals Biosciences HiScale 16/40
The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter simplifying adjustments as well as disassembly and cleaning. The measurement scale on the column makes it easier to predict and reach a predefined packing height thereby increasing process control as well as reproducibility. Although optimized for high-pressure media HiScale columns are fully compatible with all applications (and packing methods) currently applicable to XK columns.
Sigma Aldrich Fine Chemicals Biosciences Protein L from Peptostrept5MG
Protein L protein binds immunoglobulin (Ig) light chains in a wide range of species including human mouse rat pig hamster and chicken but has low affinity for Igs from guinea pig and rabbit. This bacterial protein interacts with Ig light chains without blocking the antigen binding site of the Igs . Thus protein L can be used for the detection of IgG IgM IgA IgE and IgD containingº light chains. It may also be used to detect fab and scfv fragments containingº light chains. Protein L does not bind to bovine sheep or goat Igs. Hence when bound to a solid support (sepharose) protein L may be used to directly purify monoclonal antibodies from media supplemented with BSA or fCS and also to directly purify human or mouse antibodies from goat bovine or sheep proteins. rProtein L is a recombinant form of the native protein and contains two Ig binding protein L fragments.
Sigma Aldrich Fine Chemicals Biosciences p38delta/SAPK4 Protein act
Recombinant GST fusion protein fused to full length human p38delta/SAPK4CHK3
Sigma Aldrich Fine Chemicals Biosciences AldrichR modified converti1EA
For solvents heavier than water chloroform dichloromethane and othersContinuous extraction is achieved when fresh solvent dripping (refluxing) from condenser B travels through the aqueous phase (upper layer) in extractor G. As the solvent collects at the bottom of the extractor and reaches the level of tube E it is siphoned automatically through the open PTFE stopcock D into a heated solvent reservoir attached at C.ProcedureWith PTFE stopcock D closed transfer a volume of solvent to the extractor to about the level of the stopcock. Carefully add the aqueous material making sure it does not run out the sidearm. Clamp the extractor onto a rack and attach a suitable flask half-full of solvent at C. The flask should be equipped with boiling chips or a magnetic stirring bar and a source of heat. Open stopcock D and slowly add solvent to the extractor until excess runs over to the flasks via return tube E. Attach a reflux condenser at B and apply heat to the flasks so that rapid reflux i
Sigma Aldrich Fine Chemicals Biosciences HiLoadR 16/600 SuperdexR 2
HiLoad(R) 16/600 Superdex(R) 200 prep grade are prepacked XK columns designed for high-resolution preparative gel filtration chromatography. The column is an excellent choice for separating MAbs from dimers and contaminants.Superdex(R) prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component. Steep selectivity curves give excellent resolving power for peptides and proteins in the molecular weight ranges Mr < 10 000 (Superdex(R) 30 pg) Mr 3 000 to 70 000 (Superdex(R) 75 pg) and Mr 10 000 600 000 (Superdex(R) 200 pg). The medium combines high mechanical strength with high hydrophilicity allowing high flow rates and minimal nonspecific interactions.
Sigma Aldrich Fine Chemicals Biosciences Sera MagTM SpeedBeadsTM Bl
Sera-Mag(TM) SpeedBeads(TM) and Sera-Mag(TM) Carboxylate-Modified Magnetic Particles combine a fast magnetic response time and high binding capacity with a large surface area high sensitivity stability physical integrity and fast reaction kinetics. Covalent coupling of biomolecules such as proteins and nucleic acids to carboxyl groups on the surface is easily accomplished using our standard coupling technology. The core of the particle is made by a free radical emulsion polymerization of styrene and acid monomer. One (Sera-Mag(TM)) or two (Sera-Mag(TM) SpeedBeads(TM)) layers of magnetite are then coated onto this core while the surface is modified to minimize non-specific binding of proteins.