PAN-Biotech MEM Eagle (2x) Cell Culture Medium, w: EBSS, w/o: L-Glutamine, w/o: Phenol red, w: 4.4 g/L NaHCO3 (500 ml). 2x Modification. EBSS: Contains Earle’s Balanced Salt Solution. BICARBONATE: With 4.4 g/L Sodium Bicarbonate. GLUCOSE: With glucose. GLUTAMINE: Without glutamine. HEPES: Without HEPES. PHENOL RED: Without Phenol Red. SODIUM PYRUVATE: Without Sodium Pyruvate. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH. COUNTRY OF ORIGIN: Germany. DESCRIPTION: Minimum Essential Medium (MEM), developed by Harry Eagle, is a widely used synthetic cell culture media. After finding that Eagle's Basal Medium (BME) did not meet specific nutritional requirements of normal mammalian fibroblasts and certain HeLa cells, MEM was created with higher concentrations of amino acids to more closely resemble mammalian cell protein composition. When supplemented with serum, MEM is suitable for the cultivation of a wide variety of cells grown in monolayers.