Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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| Storage Requirements | Storage of components varies. See protocol for specific instructions. |
|---|
| Assay Sensitivity | 0.1 ng/mL |
|---|---|
| Assay Range | 0.16 to 10 ng/mL |
Novus Biologicals™ Human Lactate Dehydrogenase C - Ready-To-Use ELISA Kit (Colorimetric)
Colorimetric ELISA Kit
R&D Systems™ Human Integrin alpha 5/CD49e DuoSet ELISA
For the development of sandwich ELISAs to measure natural and recombinant human Integrin α5
Invitrogen™ Rat Activin A (INHBA) ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Family | Cytokines & Receptors |
|---|---|
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 33 μL, Serum 33 μL, Supernatant 100 μL |
| Assay Sensitivity | 2 pg/mL |
| Research Discipline | Oncology |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | Activin A |
| Specificity | This ELISA pair antibody also can detect human and mouse Activin A. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Sufficient For | 96 assays |
| Calibration Range | 2 pg/mL to 400 pg/mL |
| Interassay CV | <12% |
| Gene Symbol | Inhba |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 2-400 pg/mL |
| Accession Number | P18331 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 29200 |
| Sample Type | Plasma,Serum,Supernatant |
Invitrogen™ Mouse IGFBP-6 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Protein Family | Growth Factors & Receptors |
|---|---|
| Target Species | Mouse |
| Conjugate | HRP |
| Intraassay CV | <10% |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 20 μL, Serum 20 μL, Supernatant 50 μL |
| Assay Sensitivity | 6 pg/mL |
| Research Discipline | Development |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hr 20 min |
| Assay | Solid-phase sandwich ELISA |
| Target | IGFBP6 |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Label or Dye | Biotin |
| Calibration Range | 6 pg/mL to 6000 pg/mL |
| Interassay CV | <12% |
| Gene Symbol | IGFBP6 |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 6-6000 pg/mL |
| Accession Number | P47880 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 16012 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | IGFBP-6 |
Invitrogen™ Mouse TNFR1 (Soluble) ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Mouse |
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | Tnfrsf1a |
| Total Assay Time | 4 hr 45 min |
| Assay Sensitivity | 1 pg/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 1.23-300 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P25118 |
| Regulatory Status | RUO |
| Target | TNFR1 |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Gene Alias | CD120a; CD120a antigen; FPF; hypothetical protein; metalloproteinase inhibitor 4; MGC19588; MS5; n-smase activation assoc. factor; p55; p55 TNF receptor; p55-R; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TBP1; TBPI; TIMP metallopeptidase inhibitor 4; Timp4; TIMP-4; tissue inhibitor of metalloproteinase 4; tissue inhibitor of metalloproteinases 4; TNF receptor alpha chain; TNF receptor superfamily member 1A; TNF-alphaR1; TNF-alpha-R1; TNFalpha-R1; TNFAR; TNFR; TNF-R; TNFR I; TNFR1; TNF-R1; Tnfr-1; TNFR1-d2; Tnfr-2; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRp55; Tnfrsf1a; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor superfamily member 1A, membrane form; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor; Tumor necrosis factor-binding protein 1CD120a; CD120a antigen; FPF; hypothetical protein; metalloproteinase inhibitor 4; MGC19588; MS5; n-smase activation assoc. factor; p55; p55 TNF receptor; p55-R; p60; p62; p63; sCD120a; soluble CD120a; soluble TNFR1; sTNF RI; sTNFR I; sTNFR1; sTNFRI; TBP1; TBPI; TIMP metallopeptidase inhibitor 4; Timp4; TIMP-4; tissue inhibitor of metalloproteinase 4; tissue inhibitor of metalloproteinases 4; TNF receptor alpha chain; TNF receptor superfamily member 1A; TNF-alphaR1; TNF-alpha-R1; TNFalpha-R1; TNFAR; TNFR; TNF-R; TNFR I; TNFR1; TNF-R1; Tnfr-1; TNFR1-d2; Tnfr-2; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRp55; Tnfrsf1a; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor; tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; Tumor necrosis factor receptor superfamily member 1A, membrane form; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor; Tumor necrosis factor-binding protein 1; CD120a; FPF; TNF-R; TNF-R-I; TNF-R1; TNF-R55; TNF-alphaR1; TNFAR; TNFR60; TNFRI; TNFRp55; TNFalpha-R1; Tnfr-2; Tnfr1; p55; p55-R |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 21937 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Human TIGIT ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human |
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | TIGIT |
| Total Assay Time | 4 hr 45 min |
| Assay Sensitivity | 1.5 ng/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 1.563-100 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | Q495A1 |
| Regulatory Status | RUO |
| Target | TIGIT |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Gene Alias | ENSMUSG00000071552; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; Washington University cell adhesion molecule; WUCAMENSMUSG00000071552; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; Washington University cell adhesion molecule; WUCAM; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 201633 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Mouse ACTA2 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Mouse |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10% |
| Gene Symbol | ACTA2 |
| Total Assay Time | 3 hr 30 min |
| Assay Sensitivity | 1.88 ng/mL |
| Storage Requirements | Refer to product documentation for component specific storage temperature. |
| Assay Range | 3.13-200 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P62737 |
| Regulatory Status | RUO |
| Target | Alpha-Smooth Muscle Actin |
| Kit Contents | Pre-coated 96 well plate, Standard, Biotinylated Detection Antibody, HRP Conjugate, Standard & Sample Diluent, Biotinylated Detection Antibody Diluent, HRP Conjugate Diluent, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealer,Standard,Biotinylated Detection Antibody,HRP Conjugate,Standard & Sample Diluent,Biotinylated Detection Antibody Diluent,HRP Conjugate Diluent,Wash Buffer,TMB Substrate,Stop Solution,Plate Sealer |
| Gene Alias | 0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; asma; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg0610041G09Rik; AAT6; ACT-4; acta2; acta2.L; acta2.S; actin; Actin Alpha 2 Smooth Muscle Aorta; actin alpha 2, smooth muscle; Actin Vascular Smooth Muscle; actin, alpha 2, smooth muscle, aorta; actin, alpha 2, smooth muscle, aorta L homeolog; actin, alpha 2, smooth muscle, aorta S homeolog; actin, alpha, vascular smooth muscle; actin, aortic smooth muscle; Actin, aortic smooth muscle, intermediate form; ACTSA; ACTVS; alpha actin 2; Alpha Cardiac Actin; alpha smooth muscle actin; alpha-actin; Alpha-actin-2; alpha-cardiac actin; alphaSMA; alpha-SM-actin; alpha-smooth muscle actin; Aortic Smooth Muscle; asma; a-sma; cell growth-inhibiting gene 46 protein; GIG46; Growth Inhibiting Gene 46; hypothetical protein LOC515610; LOW QUALITY PROTEIN: actin, aortic smooth muscle; mymy5; SM actin; sma; SMactin; sm-actin; SMalphaA; smooth muscle; smooth muscle alpha-actin; vascular smooth muscle alpha-actin; wu:fb63d03; XELAEV_18034370mg; |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 11475 |
| Sample Type | Cell Lysates,Plasma,Serum,Tissue Homogenates |
| Label or Dye | Biotin |
Invitrogen™ Human MCP-2/CCL8 ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Wet or Dry Ice |
|---|---|
| Immunoassay Kit Format | Sandwich ELISA Kit |
| Target Species | Human |
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | CCL8 |
| Total Assay Time | 4 hr 45 min |
| Assay Sensitivity | 1.5 pg/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 1.5-200 pg/mL |
| Test Time | 1 hr 20 min |
| Accession Number | P80075 |
| Regulatory Status | RUO |
| Target | CCL8 (MCP2) |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| Gene Alias | 1810063B20Rik; AB023418; C-C motif chemokine 8; C-C motif chemokine ligand 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; MCP-2(6-76); monocyte chemoattractant protein 2; monocyte chemoattractant protein-2; monocyte chemotactic protein 2; SCYA10; SCYA8; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); small-inducible cytokine A81810063B20Rik; AB023418; C-C motif chemokine 8; C-C motif chemokine ligand 8; CCL8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; MCP-2(6-76); monocyte chemoattractant protein 2; monocyte chemoattractant protein-2; monocyte chemotactic protein 2; SCYA10; SCYA8; small inducible cytokine A8; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2); small-inducible cytokine A8; HC14; MCP-2; MCP2; SCYA10; SCYA8 |
| Product Type | ELISA |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Gene ID (Entrez) | 6355 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |
Invitrogen™ Human B7-H2/ICOS Ligand ELISA Kit
ELISA
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10% |
| Gene Symbol | ICOSLG |
| Total Assay Time | 4 hr 45 min |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.4 ng/mL |
| Storage Requirements | Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
| Assay Range | 0.41-100 ng/mL |
| Test Time | 1 hr 20 min |
| Accession Number | O75144 |
| Regulatory Status | RUO |
| Target | CD275 (B7-H2) |
| Kit Contents | Pre-coated 96 well plate, Standard, Assay Diluent concentrate, Biotinylated Detection Antibody, SAV-HRP, Wash Buffer, Chromogen, Stop Solution, Adhesive Plate Covers,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Absorbance Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 23308 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | Biotin |